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Literature summary for 2.3.3.10 extracted from

  • Misra, I.; Wang, C.Z.; Miziorko, H.M.
    The influence of conserved aromatic residues in 3-hydroxy-3-methylglutaryl-CoA synthase (2003), J. Biol. Chem., 12, 12.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3) Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
acetoacetyl-CoA
-
Gallus gallus
0.028
-
acetyl-CoA mutant Y130F, hydrolysis partial reaction Gallus gallus
0.307
-
acetyl-CoA mutant Y376F Gallus gallus
0.565
-
acetyl-CoA mutant Y130F Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus commited step in the pathways for isoprenoid, cholesterol and ketone body production, cytosolic isoform involved in isoprenoid/cholesterol biosynthesis (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
avian
-

Purification (Commentary)

Purification (Comment) Organism
-
Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O
-
Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?
acetyl-CoA + acetoacetyl-CoA + H2O commited step in the pathways for isoprenoid, cholesterol and ketone body production, cytosolic isoform involved in isoprenoid/cholesterol biosynthesis Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
?
acetyl-CoA + H2O
-
Gallus gallus acetate + CoA
-
?