Cloned (Comment) | Organism |
---|---|
expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3) | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
acetoacetyl-CoA | - |
Gallus gallus | |
0.028 | - |
acetyl-CoA | mutant Y130F, hydrolysis partial reaction | Gallus gallus | |
0.307 | - |
acetyl-CoA | mutant Y376F | Gallus gallus | |
0.565 | - |
acetyl-CoA | mutant Y130F | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + acetoacetyl-CoA + H2O | Gallus gallus | commited step in the pathways for isoprenoid, cholesterol and ketone body production, cytosolic isoform involved in isoprenoid/cholesterol biosynthesis | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
avian | - |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Gallus gallus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | commited step in the pathways for isoprenoid, cholesterol and ketone body production, cytosolic isoform involved in isoprenoid/cholesterol biosynthesis | Gallus gallus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + H2O | - |
Gallus gallus | acetate + CoA | - |
? |