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Literature summary for 2.3.3.10 extracted from
- 3-hydroxy-3-methylglutaryl-CoA synthase. A role for glutamate 95 in general acid/base catalysis of C-C bond formation (2000), J. Biol. Chem., 275, 17946-17953.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | target of a potent antisteroidogenic drug | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
| expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3) | Gallus gallus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-carboxy-2,2,5,5,-tetramethyl-1-pyrrolidinyloxyl-CoA | - |
Gallus gallus |  |
| 3-chloropropionyl-CoA | - |
Gallus gallus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
acetoacetyl-CoA | - |
Gallus gallus | |
| 0.011 | - |
acetyl-CoA | acetyl-CoA hydrolysis, wild-type | Gallus gallus | |
| 0.012 | - |
acetoacetyl-CoA | - |
Gallus gallus | |
| 0.014 | - |
acetyl-CoA | acetyl-CoA hydrolysis, mutant E95A | Gallus gallus | |
| 0.29 | - |
acetyl-CoA | wild-type enzyme | Gallus gallus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57600 | - |
2 * 57600 | Gallus gallus |
| 116000 | - |
gel filtration | Gallus gallus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + acetoacetyl-CoA + H2O | Gallus gallus | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | Homo sapiens | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | Methanothermobacter thermautotrophicus | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | Arabidopsis thaliana | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | Blattella germanica | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | Borreliella burgdorferi | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | Mus musculus | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | Pinus sylvestris | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | Rattus norvegicus | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | Schizosaccharomyces pombe | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | Sus scrofa | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | P54873 | - |
- |
| Blattella germanica | P54870 | cockroach, cytosolic isoform 2 | - |
| Blattella germanica | P54961 | cockroach, cytosolic isoform 1 | - |
| Borreliella burgdorferi | O51626 | - |
- |
| Gallus gallus | - |
avian | - |
| Gallus gallus | P23228 | chicken, cytosolic isoform | - |
| Homo sapiens | - |
human | - |
| Homo sapiens | P54868 | human, mitochondrial isoform | - |
| Methanothermobacter thermautotrophicus | - |
- |
- |
| Mus musculus | P54869 | mouse, mitochondrial isoform | - |
| Pinus sylvestris | P93773 | pine | - |
| Rattus norvegicus | P22791 | rat, mitochondrial isoform | - |
| Schizosaccharomyces pombe | P54874 | - |
- |
| Sus scrofa | O02734 | pig, mitochondrial isoform | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Gallus gallus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Homo sapiens | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Methanothermobacter thermautotrophicus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Arabidopsis thaliana | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Blattella germanica | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Borreliella burgdorferi | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Mus musculus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Pinus sylvestris | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Rattus norvegicus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Schizosaccharomyces pombe | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Sus scrofa | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Homo sapiens |
| dimer | 2 * 57600 | Gallus gallus |
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