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Literature summary for 2.3.3.10 extracted from

  • Rokosz, L.L.; Boulton, D.A.; Butkiewicz, E.A.; Sanyal, G.; Cueto, M.A.; Lachance, P.A.; Hermes, J.D.
    Human cytoplasmic 3-hydroxy-3-methylglutaryl coenzyme A synthase: expression, purification, andcharacterization of recombinant wild-type and cys-129 mutant enzymes (1994), Arch. Biochem. Biophys., 312, 1-13.
    View publication on PubMed
Search for more literature for 2.3.3.10

Application

Application Comment Organism
medicine deficiency of the mitochondrial isoform correlates with human metabolic disease, cytosolic isoform is potentially useful target for drugs aimed at lowering cholesterol levels Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
cDNA subcloned and expressed from a T7-based vector in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl-CoA
-
 Gallus gallus
acetoacetyl-CoA
-
 Homo sapiens
acetoacetyl-CoA
-
 Rattus norvegicus
L-659,699 potent inhibitor of the recombinant enzyme Homo sapiens
L-659,699
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 acetyl-CoA
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Gallus gallus 5737
-
cytoplasm
-
 Homo sapiens 5737
-
cytoplasm
-
 Rattus norvegicus 5737
-
cytosol
-
 Gallus gallus 5829
-
cytosol
-
 Homo sapiens 5829
-
cytosol
-
 Rattus norvegicus 5829
-
mitochondrion
-
 Gallus gallus 5739
-
mitochondrion
-
 Homo sapiens 5739
-
mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Cricetulus griseus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Homo sapiens
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Rattus norvegicus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
 chinese hamster
-
Gallus gallus
-
 chicken
-
Homo sapiens
-
 human
-
Rattus norvegicus
-
 rat
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland
-
 Homo sapiens
-
adrenal gland
-
 Rattus norvegicus
-
fetus
-
 Homo sapiens
-
fibroblast
-
 Homo sapiens
-
liver
-
 Gallus gallus
-
liver
-
 Rattus norvegicus
-
ovary
-
 Cricetulus griseus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.76
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Cricetulus griseus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Homo sapiens (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Rattus norvegicus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Gallus gallus
dimer
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000537
-
 L-659,699
-
 Homo sapiens
0.012
-
 acetoacetyl-CoA
-
 Homo sapiens