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Literature summary for 2.3.3.10 extracted from
- Avian 3-hydroxy-3-methylglutaryl-CoA synthase (1993), J. Biol. Chem., 268, 12129-12135.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3) | Gallus gallus |
General Stability
| General Stability | Organism |
|---|---|
| enzyme is vulnerable to proteolysis, expression in Escherichia coli leads to an quite stable enzyme | Gallus gallus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | mitochondrial enzyme | Gallus gallus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
acetoacetyl-CoA | recombinant enzyme, hydrolase reaction | Gallus gallus | |
| 0.014 | - |
acetyl-CoA | wild-type, acetyl-CoA hydrolysis | Gallus gallus | |
| 0.27 | - |
acetyl-CoA | reombinant enzyme | Gallus gallus | |
| 0.3 | - |
acetyl-CoA | - |
Gallus gallus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Gallus gallus | 5829 | - |
| cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activity of the recombinant cytosolic isozyme ist stimulated by | Gallus gallus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57600 | - |
SDS-PAGE | Gallus gallus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + acetoacetyl-CoA + H2O | Gallus gallus | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | Rattus norvegicus | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
avian | - |
| Rattus norvegicus | - |
rat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme, expressed in Escherichia coli | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Gallus gallus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.5 | 1 | liver enzyme | Gallus gallus |
| 0.8 | 1 | recombinant enzyme | Gallus gallus |
| 1 | - |
- |
Gallus gallus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, recombinant enzyme, negligible loss of activity after 1 year | Gallus gallus |
| 4°C, recombinant enzyme, loses 50% activity after 1 year | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Gallus gallus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + acetoacetyl-CoA + H2O | - |
Rattus norvegicus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
| acetyl-CoA + H2O | in absence of co-substrate enzyme hydrolyzes acetyl-CoA | Gallus gallus | acetate + CoA | - |
? | |
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