Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) and HEK-293T cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
R600A | this HECT-domain mutant is inactive | Mus musculus |
Y763A | this HECT-domain mutant is inactive | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
x * 100000, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine | Mus musculus | - |
[ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q8C863 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography or glutathione Sepharose column chromatography and gel filtration | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is autoinhibited by an intramolecular interaction between its HECT (homologous to the E6-AP carboxy terminus domain) and two central WW domains. Ndfip1 binds these WW domains to release the HECT, allowing trans-thiolation and enzyme catalytic activity | Mus musculus | ? | - |
? | |
S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine | - |
Mus musculus | [ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
E2-E3 ligase | - |
Mus musculus |
Itch | - |
Mus musculus |