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Literature summary for 2.3.2.B8 extracted from

  • Nuber, U.; Scheffner, M.
    Identification of determinants in E2 ubiquitin-conjugating enzymes required for hect E3 ubiquitin-protein ligase interaction (1999), J. Biol. Chem., 274, 7576-7582.
    View publication on PubMed
Search for more literature for 2.3.2.B8

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens
-
 Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information generation of chimeric E2 enzymes consisting of different parts of human UbcH5 and murine UbcM3. Chimera consist of the N-terminal 62 amino acids of UbcH5 fused to the C-terminal 85 amino acids of UbcM3 and of the N-terminal 108 amino acids of UbcM3 fused to the C-terminal 85 amino acids of UbcH5, respectively. The ability to interact with E3 enzyme E6-AP maps to the C-terminal 85 amino acids of UbcH5 Mus musculus
additional information generation of chimeric E2 enzymes consisting of different parts of human UbcH5 and murine UbcM3. Chimera consist of the N-terminal 62 amino acids of UbcH5 fused to the C-terminal 85 amino acids of UbcM3 and of the N-terminal 108 amino acids of UbcM3 fused to the C-terminal 85 amino acids of UbcH5, respectively. The ability to interact with E3 enzyme E6-AP maps to the C-terminal 85 amino acids of UbcH5 and particularly to a phenylalanine residue at position 62 of UbcH5 that is conserved among the members of the UBC4/UBC5 subfamily but is not present in any of the other known E2 enzymes Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P51668 isoform UbcH5
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Mus musculus P52482 isoform UbcM3
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[ubiquitin-carrier protein UbcH5]-S-ubiquitinyl-L-cysteine + [HECT-E3-ubiquitin-carrier protein E6-AP]-L-cysteine a region of isoform UbcH5 encompassing the catalytic site cysteine residue is critical for its ability to interact with E3 enzymes E6-AP and RSP5. Of particular importance is a phenylalanine residue at position 62 of UbcH5 that is conserved among the members of the UBC4/UBC5 subfamily but is not present in any of the other known E2s Homo sapiens [ubiquitin-carrier protein UbcH5]-L-cysteine + [HECT-E3-ubiquitin-carrier protein E6-AP]-S-ubiquitinyl-L-cysteine
-
 ?
[ubiquitin-carrier protein UbcH5]-S-ubiquitinyl-L-cysteine + [HECT-E3-ubiquitin-carrier protein Rsp5]-L-cysteine a region of isoform UbcH5 encompassing the catalytic site cysteine residue is critical for its ability to interact with E3 enzymes E6-AP and RSP5. Of particular importance is a phenylalanine residue at position 62 of UbcH5 that is conserved among the members of the UBC4/UBC5 subfamily but is not present in any of the other known E2s Homo sapiens [ubiquitin-carrier protein UbcH5]-L-cysteine + [HECT-E3-ubiquitin-carrier protein Rsp5]-S-ubiquitinyl-L-cysteine
-
 ?

Synonyms

Synonyms Comment Organism
UbcH5
-
 Homo sapiens
UbcM3
-
 Mus musculus
UBE2D1
-
 Homo sapiens
UBE2E1
-
 Mus musculus