Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.2.B18 extracted from

  • Yakobov, N.; Mahmoudi, N.; Grob, G.; Yokokawa, D.; Saga, Y.; Kushiro, T.; Worrell, D.; Roy, H.; Schaller, H.; Senger, B.; Huck, L.; Riera Gascon, G.; Becker, H.D.; Fischer, F.
    RNA-dependent synthesis of ergosteryl-3beta-O-glycine in Ascomycota expands the diversity of steryl-amino acids (2022), J. Biol. Chem., 2022, 101657 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.B18

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as Maltose-binding protein fusion Aspergillus oryzae
expression in Escherichia coli as Maltose-binding protein fusionand with HA-tag Yarrowia lipolytica
expression in Escherichia coli as Maltose-binding protein fusions Aspergillus fumigatus

Protein Variants

Protein Variants Comment Organism
K321E/R322E mutation in the alpha helix, inactive Aspergillus fumigatus
K335E mutation in the alpha helix, inactive Aspergillus fumigatus
R217E/R221E mutations in alpha helix, inactive Yarrowia lipolytica
R217E/R221E/R224E/K228E mutations in alpha helix, inactive Yarrowia lipolytica
R224E/K228E mutations in alpha helix, inactive Yarrowia lipolytica
R328E mutation in the alpha helix, inactive Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + glycine + ergosterol Aspergillus fumigatus
-
 AMP + diphosphate + ergosteryl-3beta-O-glycine
-
 ?
ATP + glycine + ergosterol Aspergillus oryzae
-
 AMP + diphosphate + ergosteryl-3beta-O-glycine
-
 ?
ATP + glycine + ergosterol Yarrowia lipolytica
-
 AMP + diphosphate + ergosteryl-3beta-O-glycine
-
 ?
ATP + glycine + ergosterol Yarrowia lipolytica CLIB 122
-
 AMP + diphosphate + ergosteryl-3beta-O-glycine
-
 ?
ATP + glycine + ergosterol Aspergillus fumigatus ATCC MYA-4609
-
 AMP + diphosphate + ergosteryl-3beta-O-glycine
-
 ?
ATP + glycine + ergosterol Aspergillus oryzae ATCC 42149
-
 AMP + diphosphate + ergosteryl-3beta-O-glycine
-
 ?
ATP + glycine + tRNAGly Aspergillus fumigatus
-
 AMP + diphosphate + L-glycyl-tRNAGly
-
 ?
ATP + glycine + tRNAGly Aspergillus oryzae
-
 AMP + diphosphate + L-glycyl-tRNAGly
-
 ?
ATP + glycine + tRNAGly Yarrowia lipolytica
-
 AMP + diphosphate + L-glycyl-tRNAGly
-
 ?
ATP + glycine + tRNAGly Yarrowia lipolytica CLIB 122
-
 AMP + diphosphate + L-glycyl-tRNAGly
-
 ?
ATP + glycine + tRNAGly Aspergillus fumigatus ATCC MYA-4609
-
 AMP + diphosphate + L-glycyl-tRNAGly
-
 ?
ATP + glycine + tRNAGly Aspergillus oryzae ATCC 42149
-
 AMP + diphosphate + L-glycyl-tRNAGly
-
 ?
ergosterol + L-glycyl-tRNAGly Aspergillus fumigatus
-
 ergosteryl-3beta-O-glycine + tRNAGly
-
 ?
ergosterol + L-glycyl-tRNAGly Aspergillus oryzae
-
 ergosteryl-3beta-O-glycine + tRNAGly
-
 ?
ergosterol + L-glycyl-tRNAGly Yarrowia lipolytica
-
 ergosteryl-3beta-O-glycine + tRNAGly
-
 ?
ergosterol + L-glycyl-tRNAGly Yarrowia lipolytica CLIB 122
-
 ergosteryl-3beta-O-glycine + tRNAGly
-
 ?
ergosterol + L-glycyl-tRNAGly Aspergillus fumigatus ATCC MYA-4609
-
 ergosteryl-3beta-O-glycine + tRNAGly
-
 ?
ergosterol + L-glycyl-tRNAGly Aspergillus oryzae ATCC 42149
-
 ergosteryl-3beta-O-glycine + tRNAGly
-
 ?
additional information Aspergillus fumigatus no substrate: glycyl-adenylate ?
-
-
additional information Aspergillus oryzae no substrate: glycyl-adenylate ?
-
-
additional information Yarrowia lipolytica no substrate: glycyl-adenylate ?
-
-
additional information Yarrowia lipolytica CLIB 122 no substrate: glycyl-adenylate ?
-
-
additional information Aspergillus fumigatus ATCC MYA-4609 no substrate: glycyl-adenylate ?
-
-
additional information Aspergillus oryzae ATCC 42149 no substrate: glycyl-adenylate ?
-
-

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q4WB70 cf. EC 6.1.1.14
-
Aspergillus fumigatus ATCC MYA-4609 Q4WB70 cf. EC 6.1.1.14
-
Aspergillus oryzae Q2U0A3
-
-
Aspergillus oryzae ATCC 42149 Q2U0A3
-
-
Yarrowia lipolytica Q6C7J3 cf. EC 6.1.1.14
-
Yarrowia lipolytica CLIB 122 Q6C7J3 cf. EC 6.1.1.14
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glycine + ergosterol
-
 Aspergillus fumigatus AMP + diphosphate + ergosteryl-3beta-O-glycine
-
 ?
ATP + glycine + ergosterol
-
 Aspergillus oryzae AMP + diphosphate + ergosteryl-3beta-O-glycine
-
 ?
ATP + glycine + ergosterol
-
 Yarrowia lipolytica AMP + diphosphate + ergosteryl-3beta-O-glycine
-
 ?
ATP + glycine + ergosterol
-
 Yarrowia lipolytica CLIB 122 AMP + diphosphate + ergosteryl-3beta-O-glycine
-
 ?
ATP + glycine + ergosterol
-
 Aspergillus fumigatus ATCC MYA-4609 AMP + diphosphate + ergosteryl-3beta-O-glycine
-
 ?
ATP + glycine + ergosterol
-
 Aspergillus oryzae ATCC 42149 AMP + diphosphate + ergosteryl-3beta-O-glycine
-
 ?
ATP + glycine + tRNAGly
-
 Aspergillus fumigatus AMP + diphosphate + L-glycyl-tRNAGly
-
 ?
ATP + glycine + tRNAGly
-
 Aspergillus oryzae AMP + diphosphate + L-glycyl-tRNAGly
-
 ?
ATP + glycine + tRNAGly
-
 Yarrowia lipolytica AMP + diphosphate + L-glycyl-tRNAGly
-
 ?
ATP + glycine + tRNAGly
-
 Yarrowia lipolytica CLIB 122 AMP + diphosphate + L-glycyl-tRNAGly
-
 ?
ATP + glycine + tRNAGly
-
 Aspergillus fumigatus ATCC MYA-4609 AMP + diphosphate + L-glycyl-tRNAGly
-
 ?
ATP + glycine + tRNAGly
-
 Aspergillus oryzae ATCC 42149 AMP + diphosphate + L-glycyl-tRNAGly
-
 ?
ergosterol + L-glycyl-tRNAGly
-
 Aspergillus fumigatus ergosteryl-3beta-O-glycine + tRNAGly
-
 ?
ergosterol + L-glycyl-tRNAGly
-
 Aspergillus oryzae ergosteryl-3beta-O-glycine + tRNAGly
-
 ?
ergosterol + L-glycyl-tRNAGly
-
 Yarrowia lipolytica ergosteryl-3beta-O-glycine + tRNAGly
-
 ?
ergosterol + L-glycyl-tRNAGly
-
 Yarrowia lipolytica CLIB 122 ergosteryl-3beta-O-glycine + tRNAGly
-
 ?
ergosterol + L-glycyl-tRNAGly
-
 Aspergillus fumigatus ATCC MYA-4609 ergosteryl-3beta-O-glycine + tRNAGly
-
 ?
ergosterol + L-glycyl-tRNAGly
-
 Aspergillus oryzae ATCC 42149 ergosteryl-3beta-O-glycine + tRNAGly
-
 ?
additional information no substrate: glycyl-adenylate Aspergillus fumigatus ?
-
-
additional information no substrate: glycyl-adenylate Aspergillus oryzae ?
-
-
additional information no substrate: glycyl-adenylate Yarrowia lipolytica ?
-
-
additional information no substrate: glycyl-adenylate Yarrowia lipolytica CLIB 122 ?
-
-
additional information no substrate: glycyl-adenylate Aspergillus fumigatus ATCC MYA-4609 ?
-
-
additional information no substrate: glycyl-adenylate Aspergillus oryzae ATCC 42149 ?
-
-

Synonyms

Synonyms Comment Organism
AFUA_8g01260
-
 Aspergillus fumigatus
AO090011000521
-
 Aspergillus oryzae
DUF2156 domain-containing protein
-
 Aspergillus fumigatus
DUF2156 domain-containing protein
-
 Aspergillus oryzae
DUF2156 domain-containing protein
-
 Yarrowia lipolytica
ergosteryl-3beta-O-glycine synthase
-
 Aspergillus fumigatus
ergosteryl-3beta-O-glycine synthase
-
 Aspergillus oryzae
ergosteryl-3beta-O-glycine synthase
-
 Yarrowia lipolytica
ErgS
-
 Aspergillus fumigatus
ErgS
-
 Aspergillus oryzae
ErgS
-
 Yarrowia lipolytica
YALI0E00330g
-
 Yarrowia lipolytica

General Information

General Information Comment Organism
evolution ErgS consists of a freestanding DUF2156 domain encoded by a gene distinct from and paralogous to that of ergosteryl-3beta-O-L-aspartate synthase ErdS. The enzyme only uses Gly-tRNAGly produced by an independent glycyl-tRNA synthetase (GlyRS) to transfer glycine onto the 3beta-OH of ergosterol, producing ergosteryl-3beta-O-glycine. The ergosteryl-3beta-O-glycine synthesis pathway exists only in Ascomycota Aspergillus fumigatus
evolution ErgS consists of a freestanding DUF2156 domain encoded by a gene distinct from and paralogous to that of ergosteryl-3beta-O-L-aspartate synthase ErdS. The enzyme only uses Gly-tRNAGly produced by an independent glycyl-tRNA synthetase (GlyRS) to transfer glycine onto the 3beta-OH of ergosterol, producing ergosteryl-3beta-O-glycine. The ergosteryl-3beta-O-glycine synthesis pathway exists only in Ascomycota Aspergillus oryzae
evolution ErgS consists of a freestanding DUF2156 domain encoded by a gene distinct from and paralogous to that of ergosteryl-3beta-O-L-aspartate synthase ErdS. The enzyme only uses Gly-tRNAGly produced by an independent glycyl-tRNA synthetase (GlyRS) to transfer glycine onto the 3beta-OH of ergosterol, producing ergosteryl-3beta-O-glycine. The ergosteryl-3beta-O-glycine synthesis pathway exists only in Ascomycota Yarrowia lipolytica