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Literature summary for 2.3.2.8 extracted from

  • Kato, M.; Nozawa, Y.
    Complete purification of arginyl-tRNA:protein arginyltransferase from hog kidney and production of its antibody (1984), Anal. Biochem., 143, 361-367.
    View publication on PubMed

General Stability

General Stability Organism
phosphate, 0.04 M, stabilizes Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
additional information antibodies to hog enzyme prepared in rabbit Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm soluble Sus scrofa 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 35000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginyl-tRNAArg + acceptor protein Sus scrofa
-
tRNAArg + L-arginyl-[acceptor protein]
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
hog
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography on heparin-Sepharose and angiotensin II-Sepharose combined with affinity elution with lactalbumin Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.6
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
4°C, concentrated enzyme solution, 0.5 M phosphate-KOH-buffer, pH 7.8, 0.1 M 2-mercaptoethanol, up to 6 months Sus scrofa
4°C, purified, 0.05 M Tris-HCl buffer, pH 8.0, 0.1 M 2-mercaptoethanol, 0.09 M KCl, 0.02 M spermine, 1 week Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginyl-tRNAArg + acceptor protein
-
Sus scrofa tRNAArg + L-arginyl-[acceptor protein]
-
?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Sus scrofa