Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | requirement, 0.1 M, stabilizes the enzyme in a reduced state | Oryctolagus cuniculus | |
dithiothreitol | requirement, 0.01 M stabilizes the enzyme in a reduced state | Oryctolagus cuniculus |
General Stability | Organism |
---|---|
2-mercaptoethanol or dithiothreitol stabilize during storage | Oryctolagus cuniculus |
glycerol, 20%, stabilizes concentrated enzyme solutions during storage | Oryctolagus cuniculus |
polyethylene glycol, 30%, stabilizes concentrated solutions during storage | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | soluble | Oryctolagus cuniculus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | requirement, optimal concentration is 0.2 M | Oryctolagus cuniculus | |
K+ | requirement for monovalent cations | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginyl-tRNAArg + acceptor protein | Oryctolagus cuniculus | - |
tRNAArg + L-arginyl-[acceptor protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
7000fold | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.114 | - |
- |
Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
0°C, concentrated enzyme solution, 20% glycerol, t1/2: about 1 week | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginyl-tRNAArg + acceptor protein | - |
Oryctolagus cuniculus | tRNAArg + L-arginyl-[acceptor protein] | - |
? | |
L-arginyl-tRNAArg + albumin | from bovine serum, accepts 1 mol arginine/mol | Oryctolagus cuniculus | tRNAArg + L-arginyl-albumin | - |
? | |
L-arginyl-tRNAArg + fructose diphosphatase | from rabbit liver | Oryctolagus cuniculus | tRNAArg + L-Arg-[fructose diphosphatase] | - |
? | |
L-arginyl-tRNAArg + insulin | less effective | Oryctolagus cuniculus | tRNAArg + L-arginyl-[insulin] | - |
? | |
L-arginyl-tRNAArg + thyroglobulin | accepts 2 mol arginine/mol | Oryctolagus cuniculus | tRNAArg + L-arginyl-[thyroglobulin] | - |
? | |
L-arginyl-tRNAArg + transaldolase | I and III from Candida | Oryctolagus cuniculus | tRNAArg + L-arginyl-[transaldolase] | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | 9.4 | - |
Oryctolagus cuniculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9.8 | about half-maximal activity at pH 8.0, about 80% of maximal activity at pH 9.8 | Oryctolagus cuniculus |