Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Oryctolagus cuniculus |
General Stability | Organism |
---|---|
freezing inactivates | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by puromycin | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | soluble | Oryctolagus cuniculus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | requirement for monovalent cations | Oryctolagus cuniculus | |
additional information | inactive with Mg2+ | Oryctolagus cuniculus | |
Na+ | requirement for monovalent cations | Oryctolagus cuniculus | |
NH4+ | requirement for monovalent cations | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginyl-tRNAArg + acceptor protein | Oryctolagus cuniculus | catalyzes post-translational ribosome-independent modification of certain acceptor proteins | tRNAArg + L-arginyl-[acceptor protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
rabbit | - |
Purification (Comment) | Organism |
---|---|
partial | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00084 | - |
- |
Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-20°C, crude enzyme, at least 6 months | Oryctolagus cuniculus |
0°C, partially purified, 75% loss of activity within 1 week | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginyl-tRNAArg + acceptor protein | addition to amino-terminus | Oryctolagus cuniculus | tRNAArg + L-arginyl-[acceptor protein] | - |
? | |
L-arginyl-tRNAArg + acceptor protein | catalyzes post-translational ribosome-independent modification of certain acceptor proteins | Oryctolagus cuniculus | tRNAArg + L-arginyl-[acceptor protein] | - |
? | |
L-arginyl-tRNAArg + albumin | from bovine serum | Oryctolagus cuniculus | tRNAArg + L-arginyl-albumin | - |
? | |
L-arginyl-tRNAArg + fructose diphosphatase | from rabbit liver | Oryctolagus cuniculus | tRNAArg + L-Arg-[fructose diphosphatase] | - |
? | |
L-arginyl-tRNAArg + insulin | - |
Oryctolagus cuniculus | tRNAArg + L-arginyl-[insulin] | - |
? | |
L-arginyl-tRNAArg + thyroglobulin | - |
Oryctolagus cuniculus | tRNAArg + L-arginyl-[thyroglobulin] | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryctolagus cuniculus |