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Literature summary for 2.3.2.6 extracted from

  • Fung, A.W.; Ebhardt, H.A.; Abeysundara, H.; Moore, J.; Xu, Z.; Fahlman, R.P.
    An alternative mechanism for the catalysis of peptide bond formation by L/F transferase: substrate binding and orientation (2011), J. Mol. Biol., 409, 617-629.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Protein Variants

Protein Variants Comment Organism
D186A inactive Escherichia coli
D186E the mutation slightly reduces the reaction rate Escherichia coli
D186N inactive Escherichia coli
M144A the mutant exhibits about 9% activity compared to the wild type Escherichia coli
Q188A the mutant exhibits about 8% activity compared to the wild type Escherichia coli
W111A inactive Escherichia coli
W49A the mutant exhibits about 10% activity compared to the wild type Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-phenylalanyl-tRNA(Leu) + L-arginyl-peptide Escherichia coli
-
tRNA(Leu) + L-phenylalanyl-L-arginyl-protein
-
?
L-phenylalanyl-tRNA(Phe) + L-arginyl-peptide Escherichia coli
-
tRNA(Phe) + L-phenylalanyl-L-arginyl-protein
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A8P1
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanyl-tRNA + REPGLCTWQSLR
-
Escherichia coli t-RNA + FREPGLCTWQSLR
-
?
L-phenylalanyl-tRNA(Leu) + L-arginyl-peptide
-
Escherichia coli tRNA(Leu) + L-phenylalanyl-L-arginyl-protein
-
?
L-phenylalanyl-tRNA(Phe) + L-arginyl-peptide
-
Escherichia coli tRNA(Phe) + L-phenylalanyl-L-arginyl-protein
-
?

Synonyms

Synonyms Comment Organism
L/F transferase
-
Escherichia coli
leucyl/phenylalanyl tRNA protein transferase
-
Escherichia coli