Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D186A | inactive | Escherichia coli |
D186E | the mutation slightly reduces the reaction rate | Escherichia coli |
D186N | inactive | Escherichia coli |
M144A | the mutant exhibits about 9% activity compared to the wild type | Escherichia coli |
Q188A | the mutant exhibits about 8% activity compared to the wild type | Escherichia coli |
W111A | inactive | Escherichia coli |
W49A | the mutant exhibits about 10% activity compared to the wild type | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanyl-tRNA(Leu) + L-arginyl-peptide | Escherichia coli | - |
tRNA(Leu) + L-phenylalanyl-L-arginyl-protein | - |
? | |
L-phenylalanyl-tRNA(Phe) + L-arginyl-peptide | Escherichia coli | - |
tRNA(Phe) + L-phenylalanyl-L-arginyl-protein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A8P1 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanyl-tRNA + REPGLCTWQSLR | - |
Escherichia coli | t-RNA + FREPGLCTWQSLR | - |
? | |
L-phenylalanyl-tRNA(Leu) + L-arginyl-peptide | - |
Escherichia coli | tRNA(Leu) + L-phenylalanyl-L-arginyl-protein | - |
? | |
L-phenylalanyl-tRNA(Phe) + L-arginyl-peptide | - |
Escherichia coli | tRNA(Phe) + L-phenylalanyl-L-arginyl-protein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L/F transferase | - |
Escherichia coli |
leucyl/phenylalanyl tRNA protein transferase | - |
Escherichia coli |