Crystallization (Comment) | Organism |
---|---|
crystal structures of the Escherichia coli LF-transferase complex with phenyalanyl adenosine (rA-Phe), with or without a short peptide bearing an N-terminal Arg residue. In the presence of both the donor and acceptor substrates, the peptide formation proceedes within the crystals, and the product peptide bearing Phe at the N terminus is retained on the LF-transferase | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A8P1 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-leucyl-tRNALeu + N-terminal L-lysyl-[protein] = tRNALeu + N-terminal L-leucyl-L-lysyl-[protein] | the electron relay from Asp 186 to Gln 188 helps Gln 188 to attract a proton from the a-amino group of the N-terminal Arg of the acceptor peptide. This generates the attacking nucleophile for the carbonyl carbon of the aminoacyl bond of the aminoacyl-tRNA, thus facilitating peptide-bond formation | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
leucyl/phenylalanyl-tRNA protein transferase | - |
Escherichia coli |
LF-transferase | - |
Escherichia coli |