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Literature summary for 2.3.2.5 extracted from

  • Huang, K.F.; Liaw, S.S.; Huang, W.L.; Chia, C.Y.; Lo, Y.C.; Chen, Y.L.; Wang, A.H.
    Structures of human Golgi-resident glutaminyl cyclase and its complexes with inhibitors reveal a large loop movement upon inhibitor binding (2011), J. Biol. Chem., 286, 12439-12449.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.5

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens
expressed in Escherichia coli BL21(DE3) CodonPlus-RIL cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzyme W207F, hanging drop vapor diffusion method, using 1.7 M (NH4)2SO4, 4% (v/v) dioxane, and 100 mM MES, pH 6.5, at 25°C Homo sapiens
sitting drop vapor diffusion method, using either 30% (w/v) PEG 200, 5% (w/v) PEG 3000, and 0.1 M MES, pH 6.0, or 50% (w/v) PEG 200, 0.2 M MgCl2, and 0.1 M cacodylate, pH 6.5 Homo sapiens

Protein Variants

Protein Variants Comment Organism
W207F the mutation reduces the turnover rate (kcat) of the enzyme by about 3.7fold compared to the wild type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1-Benzylimidazole
-
 Homo sapiens
cacodylate
-
 Homo sapiens
Nomega-acetylhistamine
-
 Homo sapiens
PBD150
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Homo sapiens
-
-
Golgi apparatus
-
 Homo sapiens 5794
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ the enzyme contains one zinc ion Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens Q9NXS2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens
Ni-NTA column chromatography and Sephacryl S-100 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Gln-2-naphthylamide
-
 Homo sapiens 5-oxoprolyl-2-naphthylamide + NH3
-
 ?

Synonyms

Synonyms Comment Organism
glutaminyl cyclase
-
 Homo sapiens
Golgi-resident glutaminyl cyclase
-
 Homo sapiens
gQC
-
 Homo sapiens
sQC secretory glutaminyl cyclase Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000095
-
 PBD150 secretory glutaminyl cyclase, at 25°C in 50 mM Tris-HCl, pH 8.0 Homo sapiens
0.000262
-
 1-Benzylimidazole Golgi-resident glutaminyl cyclase, at 25°C in 50 mM Tris-HCl, pH 8.0 Homo sapiens
0.000607
-
 1-Benzylimidazole secretory glutaminyl cyclase, at 25°C in 50 mM Tris-HCl, pH 8.0 Homo sapiens
0.001698
-
 Nomega-acetylhistamine secretory glutaminyl cyclase, at 25°C in 50 mM Tris-HCl, pH 8.0 Homo sapiens
0.001817
-
 PBD150 Golgi-resident glutaminyl cyclase, at 25°C in 50 mM Tris-HCl, pH 8.0 Homo sapiens
0.005748
-
 Nomega-acetylhistamine Golgi-resident glutaminyl cyclase, at 25°C in 50 mM Tris-HCl, pH 8.0 Homo sapiens
1.824
-
 cacodylate secretory glutaminyl cyclase, at 25°C in 50 mM Tris-HCl, pH 8.0 Homo sapiens
6.696
-
 cacodylate Golgi-resident glutaminyl cyclase, at 25°C in 50 mM Tris-HCl, pH 8.0 Homo sapiens