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Literature summary for 2.3.2.5 extracted from

  • Koch, B.; Kolenko, P.; Buchholz, M.; Ruiz Carrillo, D.; Parthier, C.; Wermann, M.; Rahfeld, J.U.; Reuter, G.; Schilling, S.; Stubbs, M.T.; Demuth, H.U.
    Crystal structures of glutaminyl cyclases (QCs) from Drosophila melanogaster reveal active site conservation between insect and mammalian QCs (2012), Biochemistry, 51, 7383-7392.
    View publication on PubMed
Search for more literature for 2.3.2.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15 cells (isoform isoDromeQC) Drosophila melanogaster
expressed in Pichia pstoris strain X33 (isoform DromeQC) Drosophila melanogaster

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 10% (w/v) PEG 35000 and 100 mM Tris (pH 8.5) for isoform DromeQC Drosophila melanogaster
hanging drop vapor diffusion method, using 200 mM MnCl2, 30% (w/v) PEG 4000, and 100 mM Tris (pH 8.5) for isoform isoDromeQC Drosophila melanogaster

Protein Variants

Protein Variants Comment Organism
C113A/C136A mutant of isoform DromeQC lacking a disulfide bond, without influence on the stability of the enzyme Drosophila melanogaster
C136A/C158A mutant of isoform isoDromeQC lacking a disulfide bond, without influence on the stability of the enzyme Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
1-Methylimidazole
-
 Drosophila melanogaster
benzimidazole
-
 Drosophila melanogaster
benzylimidazole
-
 Drosophila melanogaster
imidazole
-
 Drosophila melanogaster
Nomega-acetylhistamine
-
 Drosophila melanogaster
PBD150 potent competitive inhibitor, i.e. 1-(3,4-dimethoxyphenyl)-3-(3-imidazol-1-ylpropyl)thiourea; potent competitive inhibitor, i.e. 1-(3,4-dimethoxyphenyl)-3-(3-imidazol-1-ylpropyl)thiourea Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular isoform DromeQC Drosophila melanogaster
-
-
mitochondrion isoform isoDromeQC Drosophila melanogaster 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
39SrpL21 protein Drosophila melanogaster
-
 ?
-
 ?
Acp33A protein Drosophila melanogaster
-
 ?
-
 ?
ALDH2 protein Drosophila melanogaster
-
 ?
-
 ?
DIM-10 protein Drosophila melanogaster
-
 ?
-
 ?
MCCB protein Drosophila melanogaster
-
 ?
-
 ?
NPLP4 protein Drosophila melanogaster
-
 ?
-
 ?
rho-7 protein Drosophila melanogaster
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q86PD7 isoform isoDromeQC
-
Drosophila melanogaster Q9VRQ9 isoform DromeQC
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
39SrpL21 protein
-
 Drosophila melanogaster ?
-
 ?
Acp33A protein
-
 Drosophila melanogaster ?
-
 ?
ALDH2 protein
-
 Drosophila melanogaster ?
-
 ?
DIM-10 protein
-
 Drosophila melanogaster ?
-
 ?
L-Gln-2-naphthylamide
-
 Drosophila melanogaster 5-oxoprolyl-2-naphthylamide + NH3
-
 ?
L-Gln-7-amido-4-methylcoumarin
-
 Drosophila melanogaster 5-oxoprolyl-7-amido-4-methylcoumarin + NH3
-
 ?
L-Gln-Gly
-
 Drosophila melanogaster 5-oxoprolylglycine + NH3
-
 ?
L-Gln-Gly-L-Pro
-
 Drosophila melanogaster 5-oxoprolyl-Gly-L-Pro + NH3
-
 ?
L-Gln-L-Lys-L-Arg-L-Leu
-
 Drosophila melanogaster 5-oxoprolyl-L-Lys-L-Arg-L-Leu + NH3
-
 ?
L-Gln-L-Phe-L-Ala
-
 Drosophila melanogaster 5-oxoprolyl-L-Phe-L-Ala + NH3
-
 ?
MCCB protein
-
 Drosophila melanogaster ?
-
 ?
NPLP4 protein
-
 Drosophila melanogaster ?
-
 ?
rho-7 protein
-
 Drosophila melanogaster ?
-
 ?

Synonyms

Synonyms Comment Organism
DromeQC isoform Drosophila melanogaster
glutaminyl cyclase
-
 Drosophila melanogaster
isoDromeQC isoform Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 isoform DromeQC Drosophila melanogaster
7.5 8 isoform isoDromeQC Drosophila melanogaster

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0001013
-
 PBD150 isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
0.0001093
-
 PBD150 isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
0.0003706
-
 PBD150 isoform isoDromeQC, at 30°C in 50 mM MES (pH 6.0) Drosophila melanogaster
0.000944
-
 PBD150 isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
0.00248
-
 PBD150 isoform DromeQC, at 30°C in 50 mM MES (pH 6.0) Drosophila melanogaster
0.003139
-
 PBD150 isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2
-
 NPLP4 protein isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
4.6
-
 NPLP4 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
6
-
 NPLP4 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
7.4
-
 NPLP4 protein isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
7.8
-
 ALDH2 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
8.3
-
 ALDH2 protein isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
13.9
-
 DIM-10 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
15.9
-
 39SrpL21 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
17.5
-
 DIM-10 protein isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
20.1
-
 NPLP4 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
20.2
-
 39SrpL21 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
20.9
-
 NPLP4 protein isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
20.9
-
 39SrpL21 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
21.1
-
 39SrpL21 protein isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
21.5
-
 NPLP4 protein isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
21.9
-
 39SrpL21 protein isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
22
-
 MCCB protein isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
22.8
-
 MCCB protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
23.8
-
 NPLP4 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
25
-
 MCCB protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
25.5
-
 MCCB protein isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
25.9
-
 DIM-10 protein isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
26.1
-
 MCCB protein isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
26.2
-
 rho-7 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
26.2
-
 ALDH2 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
27.6
-
 39SrpL21 protein isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
28.9
-
 MCCB protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
30.2
-
 rho-7 protein isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
31.2
-
 DIM-10 protein isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
31.2
-
 DIM-10 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
32
-
 ALDH2 protein isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
32.3
-
 MCCB protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
33.2
-
 MCCB protein isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
33.9
-
 ALDH2 protein isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
35
-
 Acp33A protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
36
-
 Acp33A protein isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
36.9
-
 Acp33A protein isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
38
-
 Acp33A protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
40.9
-
 ALDH2 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
40.9
-
 rho-7 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
43.3
-
 DIM-10 protein isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
43.4
-
 rho-7 protein isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
45.3
-
 39SrpL21 protein isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
49.9
-
 39SrpL21 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
50.2
-
 rho-7 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
50.7
-
 DIM-10 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
53.3
-
 rho-7 protein isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
55
-
 rho-7 protein isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
59.3
-
 DIM-10 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
69
-
 ALDH2 protein isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
73.1
-
 ALDH2 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
88
-
 rho-7 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster