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Literature summary for 2.3.2.5 extracted from

  • Stephan, A.; Wermann, M.; Von Bohlen, A.; Koch, B.; Cynis, H.; Demuth, H.; Schilling, S.
    Mammalian glutaminyl cyclases and their isoenzymes have identical enzymatic characteristics (2009), FEBS J., 276, 6522-6536.
    View publication on PubMed
Search for more literature for 2.3.2.5

Cloned(Commentary)

Cloned (Comment) Organism
modified N-terminal region, His-tagged version, expressed in Pichia pastoris Mus musculus
modified N-terminal region, His-tagged version, expressed in Pichia pastoris Homo sapiens

Protein Variants

Protein Variants Comment Organism
C369A improvement of enzyme secretion (expressed in Pichia pastoris) Homo sapiens
I73N artificial glycosylation site Homo sapiens
I73N/C369A further improvement of enzyme secretion (expressed in Pichia pastoris) Homo sapiens
I74N artificial glycosylation site Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
benzimidazole competitive Homo sapiens
benzimidazole competitive Mus musculus
benzylimidazole competitive Homo sapiens
benzylimidazole competitive Mus musculus
cysteamine
-
 Mus musculus
imidazole
-
 Homo sapiens
imidazole
-
 Mus musculus
L-glutaminyl-7-amido-4-methylcoumarin substrate inhibition Homo sapiens
L-glutaminyl-beta-naphthylamide substrate inhibition Homo sapiens
L-glutaminyl-beta-naphthylamide substrate inhibition Mus musculus
methylimidazole
-
 Homo sapiens
methylimidazole
-
 Mus musculus
N,N-dimethylcysteamine
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 QEYF protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
0.03
-
 L-glutaminyl-7-amido-4-methylcoumarin protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
0.03
-
 L-glutaminyl-7-amido-4-methylcoumarin protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
0.032
-
 L-glutaminyl-beta-naphthylamide protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
0.035
-
 L-glutaminyl-beta-naphthylamide protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
0.04
-
 QEYF protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
0.058
-
 Gln-Tyr-Ala protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
0.06
-
 Gln-Phe-Ala protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
0.08
-
 Gln-Tyr-Ala protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
0.092
-
 Gln-Gln protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
0.1
-
 Gln-Phe-Ala protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
0.102
-
 Gln-Gly-Pro protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
0.11
-
 Gln-Gln protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
0.16
-
 Gln-Gly protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
0.16
-
 QEDL protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
0.16
-
 QEDL protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
0.23
-
 Gln-Gly-Pro protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
0.36
-
 Gln-Gly protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
0.47
-
 Gln-Glu protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
0.61
-
 Gln-Glu protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi complex
-
 Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged version Homo sapiens
hydrophobic interaction chromatography, ion exchange chromatography, gel filtration Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gln-Gln
-
 Mus musculus pGlu-Gln + NH3
-
 ?
Gln-Gln
-
 Homo sapiens pGlu-Gln + NH3
-
 ?
Gln-Glu
-
 Mus musculus pGlu-Glu + NH3
-
 ?
Gln-Glu
-
 Homo sapiens pGlu-Glu + NH3
-
 ?
Gln-Gly
-
 Mus musculus pGlu-Gly + NH3
-
 ?
Gln-Gly
-
 Homo sapiens pGlu-Gly + NH3
-
 ?
Gln-Gly-Pro
-
 Mus musculus pGlu-Gly-Pro + NH3
-
 ?
Gln-Gly-Pro
-
 Homo sapiens pGlu-Gly-Pro + NH3
-
 ?
Gln-Phe-Ala
-
 Mus musculus pGlu-Phe-Ala + NH3
-
 ?
Gln-Phe-Ala
-
 Homo sapiens pGlu-Phe-Ala + NH3
-
 ?
Gln-Tyr-Ala
-
 Mus musculus pGlu-Tyr-Ala + NH3
-
 ?
Gln-Tyr-Ala
-
 Homo sapiens pGlu-Tyr-Ala + NH3
-
 ?
L-glutaminyl-7-amido-4-methylcoumarin
-
 Mus musculus pGlu-7-amido-4-methylcoumarin + NH3
-
 ?
L-glutaminyl-7-amido-4-methylcoumarin
-
 Homo sapiens pGlu-7-amido-4-methylcoumarin + NH3
-
 ?
L-glutaminyl-beta-naphthylamide
-
 Mus musculus pGlu-beta-naphthylamide + NH3
-
 ?
L-glutaminyl-beta-naphthylamide
-
 Homo sapiens pGlu-beta-naphthylamide + NH3
-
 ?
QEDL
-
 Mus musculus pEEDL + NH3
-
 ?
QEDL
-
 Homo sapiens pEEDL + NH3
-
 ?
QEYF
-
 Mus musculus pEEYF + NH3
-
 ?
QEYF
-
 Homo sapiens pEEYF + NH3
-
 ?

Synonyms

Synonyms Comment Organism
glutaminyl cyclase isoenzyme Mus musculus
glutaminyl cyclase isoenzyme Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.07
-
 L-glutaminyl-7-amido-4-methylcoumarin protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
1.65
-
 Gln-Gly protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.6
-
 Gln-Glu protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
2.7
-
 Gln-Gln protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
3.3
-
 QEYF protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
3.4
-
 L-glutaminyl-beta-naphthylamide protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
4
-
 Gln-Gly-Pro protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
4.57
-
 Gln-Gly protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
6.4
-
 QEDL protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
6.4
-
 QEDL protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
6.98
-
 L-glutaminyl-7-amido-4-methylcoumarin protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
7.5
-
 Gln-Phe-Ala protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
7.7
-
 Gln-Tyr-Ala protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
7.79
-
 Gln-Glu protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
8.66
-
 Gln-Gln protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
11.4
-
 Gln-Gly-Pro protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
11.78
-
 QEYF protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
17.48
-
 L-glutaminyl-beta-naphthylamide protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
22.9
-
 Gln-Tyr-Ala protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
24.1
-
 Gln-Phe-Ala protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 for kcat/KM Mus musculus
7.5 8 for kcat/KM Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0039
-
 benzylimidazole protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
0.0062
-
 benzylimidazole protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
0.027
-
 N,N-dimethylcysteamine protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
0.052
-
 methylimidazole protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
0.069
-
 cysteamine protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
0.082
-
 methylimidazole protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
0.103
-
 imidazole protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
0.124
-
 benzimidazole protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
0.235
-
 imidazole protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
0.25
-
 benzimidazole protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
1.57
-
 L-glutaminyl-beta-naphthylamide protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
3.55
-
 L-glutaminyl-beta-naphthylamide protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
4.47
-
 L-glutaminyl-7-amido-4-methylcoumarin protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9
-
 Gln-Glu protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
9
-
 Gln-Gly protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
16
-
 Gln-Glu protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
28
-
 Gln-Gly protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
38
-
 Gln-Gly-Pro protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
54
-
 Gln-Gln protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
55
-
 QEDL protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
95
-
 Gln-Gln protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
103
-
 L-glutaminyl-7-amido-4-methylcoumarin protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
104
-
 QEDL protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
111
-
 Gln-Gly-Pro protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
117
-
 Gln-Phe-Ala protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
123
-
 QEYF protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
207
-
 Gln-Tyr-Ala protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
229
-
 L-glutaminyl-beta-naphthylamide protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions Homo sapiens
311
-
 L-glutaminyl-7-amido-4-methylcoumarin protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
394
-
 Gln-Tyr-Ala protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
403
-
 Gln-Phe-Ala protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
413
-
 QEYF protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus
554
-
 L-glutaminyl-beta-naphthylamide protein expressed in Pichia pastoris, pH 8.0, 30°C Mus musculus