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Literature summary for 2.3.2.5 extracted from

  • Seifert, F.; Schulz, K.; Koch, B.; Manhart, S.; Demuth, H.U.; Schilling, S.
    Glutaminyl cyclases display significant catalytic proficiency for glutamyl substrates (2009), Biochemistry, 48, 11831-11833.
    View publication on PubMed
Search for more literature for 2.3.2.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Mus musculus
expressed in Pichia pastoris Homo sapiens
expressed in Pichia pastoris Solanum tuberosum

Inhibitors

Inhibitors Comment Organism Structure
EFRH-NH2 competitive inhibitor with L-glutaminyl-7-amido-4-methylcumarin as substrate Homo sapiens
EFRH-NH2 competitive inhibitor with L-glutaminyl-7-amido-4-methylcumarin as substrate Mus musculus
EFRHHDSGYE-NH2 competitive inhibitor with L-glutaminyl-7-amido-4-methylcumarin as substrate Homo sapiens
EFRHHDSGYE-NH2 competitive inhibitor with L-glutaminyl-7-amido-4-methylcumarin as substrate Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6
-
 EFRHHDSGYE-NH2
-
 Homo sapiens
6
-
 EFRH-NH2 at pH 6.5, 10times higher compared to KM-value of Gln substrate Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Mus musculus
Zn2+
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Carica papaya
-
-
-
Homo sapiens
-
-
-
Mus musculus
-
-
-
Solanum tuberosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus
-
 Homo sapiens
-
 Solanum tuberosum
-
 Carica papaya

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
EFRH-NH2
-
 Mus musculus pEFRH-NH2 + H2O
-
 ?
EFRH-NH2
-
 Homo sapiens pEFRH-NH2 + H2O
-
 ?
EFRH-NH2
-
 Solanum tuberosum pEFRH-NH2 + H2O
-
 ?
EFRH-NH2
-
 Carica papaya pEFRH-NH2 + H2O
-
 ?
EFRHHDSGYE-NH2
-
 Mus musculus pEFRHHDSGYE-NH2 + H2O
-
 ?
EFRHHDSGYE-NH2
-
 Homo sapiens pEFRHHDSGYE-NH2 + H2O
-
 ?
EFRHHDSGYE-NH2
-
 Solanum tuberosum pEFRHHDSGYE-NH2 + H2O
-
 ?
EFRHHDSGYE-NH2
-
 Carica papaya pEFRHHDSGYE-NH2 + H2O
-
 ?
L-glutaminyl-7-amido-4-methylcoumarin
-
 Homo sapiens pGlu-7-amido-4-methylcoumarin + NH3
-
 ?
QFRH-NH2
-
 Mus musculus pEFRH-NH2 + NH3
-
 ?
QFRH-NH2
-
 Homo sapiens pEFRH-NH2 + NH3
-
 ?
QFRH-NH2
-
 Solanum tuberosum pEFRH-NH2 + NH3
-
 ?
QFRH-NH2
-
 Carica papaya pEFRH-NH2 + NH3
-
 ?

Synonyms

Synonyms Comment Organism
glutaminyl cyclase
-
 Mus musculus
glutaminyl cyclase
-
 Homo sapiens
glutaminyl cyclase
-
 Solanum tuberosum
glutaminyl cyclase
-
 Carica papaya

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00022
-
 EFRH-NH2 at pH 6.5, 150000fold lower compared to Gln substrate Homo sapiens
0.00042
-
 EFRHHDSGYE-NH2
-
 Homo sapiens
30
-
 QFRH-NH2 at pH 6.5 Homo sapiens
40
-
 QFRH-NH2 at pH 6.5 Mus musculus
42
-
 QFRH-NH2 at pH 6.5 Carica papaya
56
-
 QFRH-NH2 at pH 6.5 Solanum tuberosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 EFRH-NH2 as substrate Homo sapiens
7.5 7.8 Gln-peptide as substrate Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.25
-
 EFRH-NH2 at pH 8.0 Homo sapiens
4.69
-
 EFRHHDSGYE-NH2 at pH 8.0 Homo sapiens
6.1
-
 EFRHHDSGYE-NH2 at pH 8.0 Mus musculus
8.32
-
 EFRHHDSGYE-NH2 at pH 6.0 Homo sapiens
8.33
-
 EFRH-NH2 at pH 8.0 Mus musculus
10.4
-
 EFRHHDSGYE-NH2 at pH 6.0 Mus musculus
24.64
-
 EFRH-NH2 at pH 6.0 Homo sapiens
35.75
-
 EFRH-NH2 at pH 6.0 Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.000019
-
 EFRH-NH2 at pH 6.5 Mus musculus
0.000037
-
 EFRH-NH2 at pH 6.5 Homo sapiens
0.000073
-
 EFRHHDSGYE-NH2 at low substrate concentrations Homo sapiens
0.00009
-
 EFRHHDSGYE-NH2
-
 Homo sapiens
0.00017
-
 EFRH-NH2 at pH 6.5 Solanum tuberosum
0.00074
-
 EFRH-NH2 at pH 6.5 Carica papaya
98.9
-
 QFRH-NH2 at pH 6.5 Solanum tuberosum
100
-
 QFRH-NH2 at pH 6.5 Homo sapiens
135
-
 QFRH-NH2 at pH 6.5 Mus musculus
145
-
 QFRH-NH2 at pH 6.5 Carica papaya