Literature summary for 2.3.2.3 extracted from

Ernst, C.M.; Staubitz, P.; Mishra, N.N.; Yang, S.J.; Hornig, G.; Kalbacher, H.; Bayer, A.S.; Kraus, D.; Peschel, A.
The bacterial defensin resistance protein MprF consists of separable domains for lipid lysinylation and antimicrobial peptide repulsion (2009), PLoS Pathog., 5, e1000660.

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Application

Application	Comment	Organism
medicine	MprF could be a target for new anti-virulence drugs	Staphylococcus aureus

Cloned(Commentary)

Cloned (Comment)	Organism
into the vector pET28	Staphylococcus aureus

Protein Variants

Protein Variants	Comment	Organism
D546A	MprF(-8) mutant, replacement results in slightly reduced production of lysyl-phosphatidylglycerol, same result is obtained when the mutation is introduced into the full-length MprF protein	Staphylococcus aureus
D731A	MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein	Staphylococcus aureus
E624A	MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein	Staphylococcus aureus
E685A	MprF(-8) mutant, replacement results in strongly reduced production of lysyl-phosphatidylglycerol, same result is obtained when the mutation is introduced into the full-length MprF protein	Staphylococcus aureus
K547A	MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein	Staphylococcus aureus
K621A	MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein	Staphylococcus aureus
K806A	MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein	Staphylococcus aureus
MprF(-10)	mutant, truncated protein consisting of residues 363-840	Staphylococcus aureus
MprF(-12)	mutant, truncated protein consisting of residues 437-840	Staphylococcus aureus
MprF(-14)	mutant, truncated protein consisting of residues 510-840	Staphylococcus aureus
MprF(-2)	mutant, truncated protein consisting of residues 84-840	Staphylococcus aureus
MprF(-4)	mutant, truncated protein consisting of residues 157-840	Staphylococcus aureus
MprF(-6)	mutant, truncated protein consisting of residues 219-840	Staphylococcus aureus
MprF(-8)	mutant, truncated protein consisting of residues 274-840	Staphylococcus aureus
MprF(-C)	mutant, truncated protein consisting of residues 1-586	Staphylococcus aureus
R734A	MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein	Staphylococcus aureus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Staphylococcus aureus	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39000	-	theoretical, MprF(-14) mutant, truncated protein consisting of residues 510-840	Staphylococcus aureus
48000	-	theoretical, MprF(-12) mutant, truncated protein consisting of residues 437-840	Staphylococcus aureus
56000	-	theoretical, MprF(-10) mutant, truncated protein consisting of residues 363-840	Staphylococcus aureus
67000	-	theoretical, MprF(-8) mutant, truncated protein consisting of residues 274-840	Staphylococcus aureus
67000	-	theoretical, MprF(-C) mutant, truncated protein consisting of residues 1-586	Staphylococcus aureus
75000	-	theoretical, MprF(-6) mutant, truncated protein consisting of residues 219-840	Staphylococcus aureus
80000	-	theoretical, MprF(-4) mutant, truncated protein consisting of residues 157-840	Staphylococcus aureus
88000	-	theoretical, MprF(-2) mutant, truncated protein consisting of residues 84-840	Staphylococcus aureus
97000	-	theoretical, MprF wild-type	Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-lysyl-tRNALys + phosphatidylglycerol	Staphylococcus aureus	-	tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol	-	?

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	Q2G2M2	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-lysyl-tRNALys + phosphatidylglycerol	-	Staphylococcus aureus	tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol	-	?

Synonyms

Synonyms	Comment	Organism
lysyltransferase	-	Staphylococcus aureus
MprF protein	-	Staphylococcus aureus
multiple peptide resistance factor protein	-	Staphylococcus aureus

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Release 2023.1 (January 2023)