Literature summary for 2.3.2.27 extracted from

Moududee, S.A.; Jiang, Y.; Gilbert, N.; Xie, G.; Xu, Z.; Wu, J.; Gong, Q.; Tang, Y.; Shi, Y.
Structural and functional characterization of hMEX-3C Ring finger domain as an E3 ubiquitin ligase (2018), Protein Sci., 27, 1661-1669 .

Search for more literature for 2.3.2.27

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure of dimeric Ring finger domain of MEX3C and comparison with the complex structure of MDM2/MDMX-UbcH5b-Ub. The Ring finger domain of MEX3C acts as a ubiquitin E3 ligase in vitro, cooperating with E2 enzymes UbcH5b and UbcH5c to mediate ubiquitination	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
C608S/C611S	mutation in Zn2+ coordination site, almost complete loss ofactivity	Homo sapiens
C632S	mutation in Zn2+ coordination site, almost complete loss of activity	Homo sapiens
I610A	mutation abolishes selfubiquitination activity. Residue may play key role to interact with E2 and stabilize ubiquitin in the closed active E2-Ub conformation	Homo sapiens
I636A	mutation abolishes selfubiquitination activity	Homo sapiens
K639A	mutation reduces selfubiquitination activity	Homo sapiens
P645A	mutation abolishes selfubiquitination activity	Homo sapiens
Q648A	mutation abolishes selfubiquitination activity. Residue may play key role to interact with E2 and stabilize ubiquitin in the closed active E2-Ub conformation	Homo sapiens
V609A	mutation reduces selfubiquitination activity	Homo sapiens
V609E	mutation abolishes selfubiquitination activity	Homo sapiens
V646E	mutation abolishes selfubiquitination activity	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
DTPA	treatment leads to inactivation	Homo sapiens
EDTA	treatment leads to inactivation	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	absolutely required	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q5U5Q3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[E2 ubiquitin-conjugating enzyme UbcH5b]-S-ubiquitinyl-L-cysteine + [Ring finger domain of MEX3C]-L-lysine	-	Homo sapiens	[E2 ubiquitin-conjugating enzyme UbcH5b]-L-cysteine + [Ring finger domain of MEX3C]-N6-ubiquitinyl-L-lysine	-	?

Synonyms

Synonyms	Comment	Organism
MEX3C	-	Homo sapiens

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Release 2023.1 (January 2023)