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Literature summary for 2.3.2.27 extracted from
- Arabidopsis C3HC4-RING finger E3 ubiquitin ligase AtAIRP4 positively regulates stress-responsive abscisic acid signaling (2016), J. Integr. Plant Biol., 58, 67-80.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta cells | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Arabidopsis thaliana | 5737 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine | Arabidopsis thaliana | - |
[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione Sepharose bead chromatography | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine | - |
Arabidopsis thaliana | [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| abscisic acid-insensitive RING protein 4 | - |
Arabidopsis thaliana |
| AIRP4 | - |
Arabidopsis thaliana |
| C3HC4-RING finger E3 ubiquitin ligase | - |
Arabidopsis thaliana |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Arabidopsis thaliana | the transcription level can be induced by absisic acid (0.1 mM), heat (37°C), osmotic (300 mM mannitol) and oxidative stresses (20 mM H2O2) | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme acts as a positive regulator of absisic acid-mediated drought avoidance and a negative regulator of salt tolerance in Arabidopsis. Enzyme overexpression plants are hypersensitive to salt and osmotic stresses during seed germination, and show drought avoidance | Arabidopsis thaliana |
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