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Literature summary for 2.3.2.27 extracted from

  • Nguyen, L.; Plafker, K.S.; Starnes, A.; Cook, M.; Klevit, R.E.; Plafker, S.M.
    The ubiquitin-conjugating enzyme, UbcM2, is restricted to monoubiquitylation by a two-fold mechanism that involves backside residues of E2 and Lys48 of ubiquitin (2014), Biochemistry, 53, 4004-4014.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information a truncation mutant spanning residues 1-360 and containing the RING finger domain is catalytically active Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96BH1
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information in combination with E2 ubiquitin-conjugating enzyme UbcM2, only monoubiquitylation of the final substrate occurs. In presence of ubiquitin mutant K48R and UbcM2, enhanced polyubiquitin synthesizing activity is found. Reaction mixtures containing ubiquitin mutant K6R show a mild suppression of UbcM2 activity Homo sapiens ?
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?
S-ubiquitinyl-[UbcM2]-L-cysteine + [acceptor protein]-L-lysine
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Homo sapiens [UbcM2]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
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?

Synonyms

Synonyms Comment Organism
E3 ubiquitin-protein ligase RNF25
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Homo sapiens