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Literature summary for 2.3.2.27 extracted from

  • Huang, F.; Xiao, H.; Sun, B.L.; Yang, R.G.
    Characterization of TRIM62 as a RING finger E3 ubiquitin ligase and its subcellular localization (2013), Biochem. Biophys. Res. Commun., 432, 208-213.
    View publication on PubMed
Search for more literature for 2.3.2.27

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C11A mutation introduced to reduce the stability of the RING domain, poor activity Homo sapiens
additional information deletion of the RING domain reulsts in loss of E3 transferase activity Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40686
-
 x * 40686, calculated Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8GYT9
-
-
Homo sapiens Q9BVG3
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293T cell
-
 Homo sapiens
-
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-ubiquitinyl-[Ubc8]-L-cysteine + [acceptor protein]-L-lysine
-
 Arabidopsis thaliana [Ubc8]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine the RING domain of Sis3 is sufficient for E3 ligase activity ?
S-ubiquitinyl-[UbcH5]-L-cysteine + [TRIM62]-L-lysine
-
 Homo sapiens [UbsH5B]-L-cysteine + N6-ubiquitinyl-[TRIM62]-L-lysine TRIM62, in association with the E2 enzyme UbcH5B, catalyzes self-ubiquitination in vitro. The process requires an intact RING finger domain. The treatment of HEK-293T cells with a proteasome inhibitor stabilizes poly-ubiquitinated TRIM62, indicating that self-ubiquitination promotes the proteasomal degradation of TRIM62 ?

Subunits

Subunits Comment Organism
? x * 40686, calculated Arabidopsis thaliana
More Sis3 sequence is predicted to have a RING domain located between amino acids 235 and 275 Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
DEAR1
-
 Homo sapiens
Sis3
-
 Arabidopsis thaliana
TRIM62
-
 Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana calculated
-
 6.3

General Information

General Information Comment Organism
physiological function mutant sugar-insensitive3, i.e. Sis3 is resistant to the inhibitory effects of high concentrations of exogenous glucose and sucrose on early seedling development. In contrast to wild-type plants, sis3 mutants develop green, expanded cotyledons and true leaves when sown on medium containing high concentrations (e.g. 270 mM) of sucrose. Mutant Sis3 exhibits wild-type responses to the inhibitory effects of abscisic acid and paclobutrazol, a gibberellic acid biosynthesis inhibitor, on seed germination Arabidopsis thaliana