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Literature summary for 2.3.2.27 extracted from

  • Hassink, G.; Kikkert, M.; Van Voorden, S.; Lee, S.; Spaapen, R.; Van Laar, T.; Coleman, C.; Bartee, E.; Früh, K.; Chau, V.; Wiertz, E.
    TEB4 is a C4HC3 RING finger-containing ubiquitin ligase of the endoplasmic reticulum (2005), Biochem. J., 388, 647-655.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.27

Cloned(Commentary)

Cloned (Comment) Organism
expression in HeLa cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C9A mutation within the RING domain of TEB4, strongly impairs its own degradation Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-
membrane sequence contains 13 predicted transmembrane domains. The N-terminal region of TEB4 is located in the cytoplasm, whereas the C-terminus of TEB4 has a luminal deposition Homo sapiens 16020
-
additional information isoform TEB4 is notexpressed at the cell surface Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
 x * 97000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O60337 isoform TEB4
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[TEB4-UBC7]-S-ubiquitinyl-L-cysteine + ubiquitin-L-lysine48
-
 Homo sapiens [TEB4-UBC7]-L-cysteine + ubiquitin-N6-ubiquitinyl-L-lysine48 formation of a ubiquitin dimer, where residue lysine48 is linked to the C-terminal carboxyl of another ubiquitin. The UBC7-dependent ubiquitin–ubiquitin linkage reaction requires the presence of the ubiquitin-activating enzyme E1 and ATP, suggesting that the activity requires the intermediate formation of an UBC7-ubiquitin thioester ?
[TEB4-UBC7]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine
-
 Homo sapiens [TEB4-UBC7]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine the isolated TEB4 RING domain catalyses ubiquitin ligation in vitro in a reaction that is ubiquitin Lys48-specific and involves ubiquitin-conjugating enzyme UBC7 ?

Subunits

Subunits Comment Organism
? x * 97000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
MARCH6
-
 Homo sapiens
TEB4
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens TEB4 expression does not increase in response to the various stress conditions additional information

General Information

General Information Comment Organism
metabolism isoform TEB4 is an ER degradation substrate itself, promoting its own degradation in a RING finger- and proteasome-dependent manner Homo sapiens