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Literature summary for 2.3.2.27 extracted from

  • Lee, D.H.; Choi, H.W.; Hwang, B.K.
    The pepper E3 ubiquitin ligase RING1 gene, CaRING1, is required for cell death and the salicylic acid-dependent defense response (2011), Plant Physiol., 156, 2011-2025.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli (BL21) as a fusion protein with maltose-binding protein MBP Capsicum annuum

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
Capsicum annuum 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20570
-
calculated Capsicum annuum

Organism

Organism UniProt Comment Textmining
Capsicum annuum G0T3B3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant fusion protein CaRING1-maltose-binding protein MBP, cell lysis, affinity chromatography of the the soluble fraction Capsicum annuum

Source Tissue

Source Tissue Comment Organism Textmining
leaf expression and ubiquitination of CaRING1 are required to induce cell death effectively in pepper leaves as a pathogen infection response Capsicum annuum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information ubiquitination activity of the fusion protein CaRING1-maltose-binding protein MBP are not displayed in the absence of E1, E2, E3, ATP or ubiquitin Capsicum annuum ? ubiquitination activity of the fusion protein CaRING1-maltose-binding protein MBP is detected using anti-ubiquitin and anti-MBP antibody ?

Synonyms

Synonyms Comment Organism
CaRING1 gene name, the RING domain belongs to the C3H2C3-type RING-H2 group which are essential for E3 ubiquitin ligase activity in the ubiquitin/26S proteasome system. The complete RING domain is essentiel for ubiquitination activity Capsicum annuum
E3 ubiquitin ligase RING1
-
Capsicum annuum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Capsicum annuum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Capsicum annuum

pI Value

Organism Comment pI Value Maximum pI Value
Capsicum annuum calculated
-
8.86

Expression

Organism Comment Expression
Capsicum annuum expression is induced by avirulent Xanthomonas campestris pv vesicatoria infection up

General Information

General Information Comment Organism
physiological function essential for hypersensitive response (HR) production. The CaRING1 expression triggers the hypersensitive cell death response. Transgenic Arabidopsis plants overexpressing CaRING1 also exhibit enhanced bacterial and fungal disease resistance, accompanied by the induction of salicylic acid against pathogen infection Capsicum annuum