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Literature summary for 2.3.2.23 extracted from

  • Kanack, A.; Vittal, V.; Haver, H.; Keppel, T.; Gundry, R.L.; Klevit, R.E.; Scaglione, K.M.
    UbcH5 interacts with substrates to participate in lysine selection with the E3 ubiquitin ligase CHIP (2020), Biochemistry, 59, 2078-2088.
    View publication on PubMed
Search for more literature for 2.3.2.23

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2203
-
 [UBE2W]-S-ubiquitinyl-L-cysteine 37°C, pH not specified in the publication Homo sapiens
0.3014
-
 [UBE2W]-S-ubiquitinyl-L-cysteine presence of the U-box domain of E3 enzyme CHIP, 37°C, pH not specified in the publication Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P51668 cf. EC 2.3.2.24
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[UBE2W]-S-ubiquitinyl-L-cysteine + [Josephin domain of ATXN3]-L-lysine
-
 Homo sapiens [UBE2W]-L-cysteine + [Josephin domain of ATXN3]-N6-ubiquitinyl-L-lysine
-
 ?

Synonyms

Synonyms Comment Organism
UbcH5
-
 Homo sapiens
UBE2D1
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.016
-
 [UBE2W]-S-ubiquitinyl-L-cysteine 37°C, pH not specified in the publication Homo sapiens
0.375
-
 [UBE2W]-S-ubiquitinyl-L-cysteine presence of the U-box domain of E3 enzyme CHIP, 37°C, pH not specified in the publication Homo sapiens

General Information

General Information Comment Organism
additional information UbcH5 selects substrate lysine residues independent of the E3 ubiquitin ligase C-terminus of Hsc70 interacting protein CHIP. CHIP participates in lysine selection by fine-tuning the subset of substrate lysines that are ubiquitinated. Residue lysine 128 near the C-terminus of UbcH5 is critical for the efficient ubiquitin transfer by UbcH5 in both the presence and absence of CHIP Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.074
-
 [UBE2W]-S-ubiquitinyl-L-cysteine 37°C, pH not specified in the publication Homo sapiens
1.244
-
 [UBE2W]-S-ubiquitinyl-L-cysteine presence of the U-box domain of E3 enzyme CHIP, 37°C, pH not specified in the publication Homo sapiens