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Literature summary for 2.3.2.23 extracted from

  • Wee, K.E.; Lai, Z.; Auger, K.R.; Ma, J.; Horiuchi, K.Y.; Dowling, R.L.; Dougherty, C.S.; Corman, J.I.; Wynn, R.; Copeland, R.A.
    Steady-state kinetic analysis of human ubiquitin-activating enzyme (E1) using a fluorescently labeled ubiquitin substrate (2000), J. Protein Chem., 19, 489-498.
    View publication on PubMed
Search for more literature for 2.3.2.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in baculoviral system Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0019
-
 [ubiquitin carrier protein Ubc4]-L-cysteine pH 7.5, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P62837 isoform Ubc4
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[ubiquitin-activating protein E1]-S-ubiquitinyl-L-cysteine + [ubiquitin carrier protein Ubc4]-L-cysteine binding of Ubc4 to the E1–ubiquitin covalent intermediate leads to productive catalysis of ubiquitin transfer to Ubc4 in the form of a thioester linkage. No significant ubiquitination of Ubc4 through formation of lysyl isopeptide bonds is observed Homo sapiens [ubiquitin-activating protein E1]-L-cysteine + [ubiquitin carrier protein Ubc4]-S-ubiquitinyl-L-cysteine
-
 ?

Synonyms

Synonyms Comment Organism
Ubc4
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.072
-
 [ubiquitin carrier protein Ubc4]-L-cysteine pH 7.5, 25°C Homo sapiens