Literature summary for 2.3.2.23 extracted from

Tokgoez, Z.; Siepmann, T.J.; Streich, F.; Kumar, B.; Klein, J.M.; Haas, A.L.
E1-E2 interactions in ubiquitin and Nedd8 ligation pathways (2012), J. Biol. Chem., 287, 311-321.

Search for more literature for 2.3.2.23

Cloned(Commentary)

Cloned (Comment)	Organism
-	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
DELTA946	truncation of the Uba3 carboxyl-terminal beta-grasp domain, no effect on cognate Ubc12 thiolester formation	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P22314	-	-
Homo sapiens	Q8TBC4	isoform Uba3	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	functional survey of 11 representative human E2 paralogs reveals similar Km values for binding to human Uba1 ternary complex with an average Km of 121 nM and kcat for ubiquitin transfer of 4.0 per s, suggesting that they possess a conserved binding site and transition state geometry and that they compete for charging through differences in intracellular concentration. This binding motif is localized to three basic residues within Helix 1 of the E2 core domain	Homo sapiens	?	-	?
[ubiquitin-activating protein Uba1]-S-ubiquitinyl-L-cysteine + [ubiquitin-carrier-protein Ubc2b]-L-cysteine	-	Homo sapiens	[ubiquitin-activating protein Uba1]-L-cysteine + [ubiquitin-carrier-protein Ubc2b]-S-ubiquitinyl-L-cysteine	-	?
[ubiquitin-activating protein Uba3]-S-ubiquitinyl-L-cysteine + [ubiquitin-carrier-protein Ubc12]-L-cysteine	-	Homo sapiens	[ubiquitin-activating protein Uba3]-L-cysteine + [ubiquitin-carrier-protein Ubc12]-S-ubiquitinyl-L-cysteine	-	?

Synonyms

Synonyms	Comment	Organism
NEDD8-activating enzyme E1 catalytic subunit	-	Homo sapiens
Uba3	-	Homo sapiens

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Release 2023.1 (January 2023)