Cloned (Comment) | Organism |
---|---|
expressed and transformed into Escherichia coli (BL-21 DE3) using pET22 vector in frame to a C-terminal His6 tag | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein, Ni-NTA column | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine | E2 enzymes autoubiquitinate both their active sites cysteine, described for this enzyme and lysine residues | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | polyubiquitin chain formation catalyzed by E2 enzymes, in the absence of an E3 protein and a target protein substrate | Homo sapiens | ? | a thiol ester-linked ubiquitin to the E2 active site is an intermediate in any polyubiquination reactions | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | E2 enzymes spontaneously dimerize in solution, in vitro, in absence of charged ubiquitin | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
E2 ubiquitin-conjugating enzyme | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at, polyubiquitination | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at, polyubiquitination | Homo sapiens |