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Literature summary for 2.3.2.23 extracted from

  • David, Y.; Ziv, T.; Admon, A.; Navon, A.
    The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines (2010), J. Biol. Chem., 285, 8595-8604.
    View publication on PubMed
Search for more literature for 2.3.2.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed and transformed into Escherichia coli (BL-21 DE3) using pET22 vector in frame to a C-terminal His6 tag Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, Ni-NTA column Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine E2 enzymes autoubiquitinate both their active sites cysteine, described for this enzyme and lysine residues Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information polyubiquitin chain formation catalyzed by E2 enzymes, in the absence of an E3 protein and a target protein substrate Homo sapiens ? a thiol ester-linked ubiquitin to the E2 active site is an intermediate in any polyubiquination reactions ?

Subunits

Subunits Comment Organism
dimer E2 enzymes spontaneously dimerize in solution, in vitro, in absence of charged ubiquitin Homo sapiens

Synonyms

Synonyms Comment Organism
E2 ubiquitin-conjugating enzyme
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at, polyubiquitination Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at, polyubiquitination Homo sapiens