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Literature summary for 2.3.2.23 extracted from

  • Li, D.F.; Feng, L.; Hou, Y.J.; Liu, W.
    The expression, purification and crystallization of a ubiquitin-conjugating enzyme E2 from Agrocybe aegerita underscore the impactof His-tag location on recombinant protein properties (2013), Acta Crystallogr. Sect. F, 69, 153-157.
    View publication on PubMed
Search for more literature for 2.3.2.23

Application

Application Comment Organism
drug development UbcA1 shows antitumor properties, a potential anticancer drug candidate Cyclocybe aegerita

Cloned(Commentary)

Cloned (Comment) Organism
subcloned in Escherichia coli BL21(DE2), via pET28 vector, N-terminally His-tagged Cyclocybe aegerita

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant enzyme, N-terminally His-tagged, subcloned in Escherichia coli BL21(DE2), via pET28 vector, purified using Ni-NTA column and HiLoad 16/60 Superdex 75 gel filtration column, diffracted X-rays to 1.7 A. The crystal belongs to space group C2 Cyclocybe aegerita

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 2 * 18000, single band on SDS-PAGE, indicating a dimeric form. Gel filtration results in an estimated molecular weight of 42 kDa Cyclocybe aegerita
42000
-
 gel filtration results in an estimated molecular weight of 42 kDa Cyclocybe aegerita

Organism

Organism UniProt Comment Textmining
Cyclocybe aegerita
-
 medicinal mushroom, basidiomycetes
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information ubiquitination is involved in myriad cell and disease pathways. The ubiquitin-conjugating E2 is the central player in the ubiquitin-transfer pathway Cyclocybe aegerita

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, N-terminally His-tagged, subcloned in Escherichia coli BL21(DE2), via pET28 vector, purified using Ni-NTA column and HiLoad 16/60 Superdex 75 gel filtration column, purity 95% after two steps Cyclocybe aegerita

Subunits

Subunits Comment Organism
dimer 2 * 18000, single band on SDS-PAGE, indicating a dimeric form. Gel filtration results in an estimated molecular weight of 42 kDa Cyclocybe aegerita

Synonyms

Synonyms Comment Organism
UbcA1
-
 Cyclocybe aegerita
ubiquitin-conjugating enzyme E2
-
 Cyclocybe aegerita

General Information

General Information Comment Organism
physiological function ubiquitination is involved in myriad cell and disease pathways. The ubiquitin-conjugating E2 is the central player in the ubiquitin-transfer pathway Cyclocybe aegerita