Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucyl-tRNALeu + L-leucyl-tRNALeu | Bacillus spizizenii | - |
tRNALeu + tRNALeu + cyclo(L-leucyl-L-leucyl) | - |
? | |
L-leucyl-tRNALeu + L-leucyl-tRNALeu | Bacillus spizizenii W23 | - |
tRNALeu + tRNALeu + cyclo(L-leucyl-L-leucyl) | - |
? | |
L-leucyl-tRNALeu + L-leucyl-tRNALeu | Bacillus spizizenii NRRL B-14472 | - |
tRNALeu + tRNALeu + cyclo(L-leucyl-L-leucyl) | - |
? | |
L-leucyl-tRNALeu + L-leucyl-tRNALeu | Bacillus spizizenii ATCC 23059 | - |
tRNALeu + tRNALeu + cyclo(L-leucyl-L-leucyl) | - |
? | |
additional information | Bacillus spizizenii | the CDPS YvmC catalyzes formation of major product cyclo(L-leucyl-L-leucyl) as well as minor cyclic dipeptide products containing one leucine residue | ? | - |
- |
|
additional information | Bacillus spizizenii W23 | the CDPS YvmC catalyzes formation of major product cyclo(L-leucyl-L-leucyl) as well as minor cyclic dipeptide products containing one leucine residue | ? | - |
- |
|
additional information | Bacillus spizizenii NRRL B-14472 | the CDPS YvmC catalyzes formation of major product cyclo(L-leucyl-L-leucyl) as well as minor cyclic dipeptide products containing one leucine residue | ? | - |
- |
|
additional information | Bacillus spizizenii ATCC 23059 | the CDPS YvmC catalyzes formation of major product cyclo(L-leucyl-L-leucyl) as well as minor cyclic dipeptide products containing one leucine residue | ? | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus spizizenii | E0U3N2 | - |
- |
Bacillus spizizenii ATCC 23059 | E0U3N2 | - |
- |
Bacillus spizizenii NRRL B-14472 | E0U3N2 | - |
- |
Bacillus spizizenii W23 | E0U3N2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucyl-tRNALeu + L-leucyl-tRNALeu | - |
Bacillus spizizenii | tRNALeu + tRNALeu + cyclo(L-leucyl-L-leucyl) | - |
? | |
L-leucyl-tRNALeu + L-leucyl-tRNALeu | - |
Bacillus spizizenii W23 | tRNALeu + tRNALeu + cyclo(L-leucyl-L-leucyl) | - |
? | |
L-leucyl-tRNALeu + L-leucyl-tRNALeu | - |
Bacillus spizizenii NRRL B-14472 | tRNALeu + tRNALeu + cyclo(L-leucyl-L-leucyl) | - |
? | |
L-leucyl-tRNALeu + L-leucyl-tRNALeu | - |
Bacillus spizizenii ATCC 23059 | tRNALeu + tRNALeu + cyclo(L-leucyl-L-leucyl) | - |
? | |
additional information | the CDPS YvmC catalyzes formation of major product cyclo(L-leucyl-L-leucyl) as well as minor cyclic dipeptide products containing one leucine residue | Bacillus spizizenii | ? | - |
- |
|
additional information | the CDPS YvmC catalyzes formation of major product cyclo(L-leucyl-L-leucyl) as well as minor cyclic dipeptide products containing one leucine residue | Bacillus spizizenii W23 | ? | - |
- |
|
additional information | the CDPS YvmC catalyzes formation of major product cyclo(L-leucyl-L-leucyl) as well as minor cyclic dipeptide products containing one leucine residue | Bacillus spizizenii NRRL B-14472 | ? | - |
- |
|
additional information | the CDPS YvmC catalyzes formation of major product cyclo(L-leucyl-L-leucyl) as well as minor cyclic dipeptide products containing one leucine residue | Bacillus spizizenii ATCC 23059 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
CDPS | - |
Bacillus spizizenii |
cyclodipeptide synthase | - |
Bacillus spizizenii |
YvmC | - |
Bacillus spizizenii |
General Information | Comment | Organism |
---|---|---|
metabolism | comparison of different CDPS-containing biosynthetic pathways, enzyme YvmC is involved in the pulcherrimin biosynthetic pathway, overview. In Bacillus subtilis, the CDPS YvmC catalyzes formation of major product cyclo(L-leucyl-L-leucyl) as well as minor cyclic dipeptide products containing one leucine residue. The yvmC gene is part of a two gene operon with cypX | Bacillus spizizenii |
additional information | the CDPS catalytic mechanism entails initial covalent tethering of the aminoacyl moiety from the first aa-tRNA substrate onto a conserved active site serine (Ser) residue. Nucleophilic attack of the amino nitrogen on the carbonyl carbon from the second aa-tRNA substrate yields the first peptide bond. The resulting enzyme-linked dipeptidyl intermediate then undergoes intramolecular peptide bond formation to yield the DKP group with concomitant release from the active site. The two aa-tRNA substrates bind at different sites of the CDPS | Bacillus spizizenii |
physiological function | cyclodipeptide synthases (CDPSs) are recognized catalysts of 2,5-diketopiperazine (DKP) assembly, employing two aminoacyl-tRNAs (aa-tRNAs) as substrates. Representative 2,5-diketopiperazine (DKP) natural products and bioactivities, overview | Bacillus spizizenii |