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Literature summary for 2.3.2.2 extracted from

  • Lee, J.; Lee, J.; Nam, G.; Son, B.; Jang, M.; Lee, S.; Hurh, B.; Kim, T.
    Heterologous expression and enzymatic characterization of gamma-glutamyltranspeptidase from Bacillus amyloliquefaciens (2017), J. Microbiol., 55, 147-152 .
    View publication on PubMed
Search for more literature for 2.3.2.2

Cloned(Commentary)

Cloned (Comment) Organism
gene BaGGT469, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of extracellular C-terminally His6-tagged enzyme in Bacillus subtilis strain KCTC 3135 and in Escherichia coli strain BL21(DE3) Bacillus amyloliquefaciens
gene BaGGT469, sequence comparisons, recombinant expression of extracellular C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Bacillus licheniformis
gene BaGGT469, sequence comparisons, recombinant expression of extracellular C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Bacillus velezensis
gene BsGGT168, sequence comparisons, recombinant expression of extracellular C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus licheniformis
-
-
extracellular
-
 Bacillus subtilis
-
-
extracellular
-
 Bacillus amyloliquefaciens
-
-
extracellular
-
 Bacillus velezensis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64509
-
 sequence calculation Bacillus amyloliquefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a (5-L-glutamyl)-peptide + an amino acid Bacillus licheniformis
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid Bacillus subtilis
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid Bacillus amyloliquefaciens
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid Bacillus velezensis
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid Bacillus subtilis 168
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid Bacillus licheniformis NCIMB 9375
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid Bacillus licheniformis Gibson 46
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid Bacillus licheniformis JCM 2505
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid Bacillus licheniformis NRRL NRS-1264
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid Bacillus velezensis BGSC 10A6
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid Bacillus velezensis DSM 23117
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid Bacillus amyloliquefaciens SMB469
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid Bacillus licheniformis NBRC 12200
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid Bacillus licheniformis ATCC 14580
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid Bacillus velezensis FZB42
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid Bacillus licheniformis DSM 13
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens A0A1S5V3K5 Bacillus amyloliquefaciens strain SMB469 with high GGT activity is isolated from a Korean traditional fermented soy food, Doenjang
-
Bacillus amyloliquefaciens SMB469 A0A1S5V3K5 Bacillus amyloliquefaciens strain SMB469 with high GGT activity is isolated from a Korean traditional fermented soy food, Doenjang
-
Bacillus licheniformis Q65KZ6
-
-
Bacillus licheniformis ATCC 14580 Q65KZ6
-
-
Bacillus licheniformis DSM 13 Q65KZ6
-
-
Bacillus licheniformis Gibson 46 Q65KZ6
-
-
Bacillus licheniformis JCM 2505 Q65KZ6
-
-
Bacillus licheniformis NBRC 12200 Q65KZ6
-
-
Bacillus licheniformis NCIMB 9375 Q65KZ6
-
-
Bacillus licheniformis NRRL NRS-1264 Q65KZ6
-
-
Bacillus subtilis P54422
-
-
Bacillus subtilis 168 P54422
-
-
Bacillus velezensis A7Z5E0
-
-
Bacillus velezensis BGSC 10A6 A7Z5E0
-
-
Bacillus velezensis DSM 23117 A7Z5E0
-
-
Bacillus velezensis FZB42 A7Z5E0
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the GGT heterodimeric enzyme is generated via autocatalytic cleavage from a precursor Bacillus licheniformis
proteolytic modification the GGT heterodimeric enzyme is generated via autocatalytic cleavage from a precursor Bacillus subtilis
proteolytic modification the GGT heterodimeric enzyme is generated via autocatalytic cleavage from a precursor Bacillus velezensis
proteolytic modification the two separate polypeptide chains of 45.7 and 19.7 kDa forming the GGT heterodimeric enzyme are generated via autocatalytic cleavage from a precursor Bacillus amyloliquefaciens

Purification (Commentary)

Purification (Comment) Organism
recombinant extracellular C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Bacillus licheniformis
recombinant extracellular C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Bacillus subtilis
recombinant extracellular C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Bacillus amyloliquefaciens
recombinant extracellular C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Bacillus velezensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.4
-
 purified recombinant His6-tagged enzyme, substrates L-gamma-glutamyl-4-nitroanilide and glycylglycine, pH 9.0, 40°C Bacillus licheniformis
12.9
-
 purified recombinant His6-tagged enzyme, substrates L-gamma-glutamyl-4-nitroanilide and glycylglycine, pH 9.0, 40°C Bacillus subtilis
16.1
-
 purified recombinant His6-tagged enzyme, substrates L-gamma-glutamyl-4-nitroanilide and glycylglycine, pH 9.0, 40°C Bacillus velezensis
17.8
-
 purified recombinant His6-tagged enzyme, substrates L-gamma-glutamyl-4-nitroanilide and glycylglycine, pH 9.0, 40°C Bacillus amyloliquefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus licheniformis a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus subtilis a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus amyloliquefaciens a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus velezensis a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus subtilis 168 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus licheniformis NCIMB 9375 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus licheniformis Gibson 46 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus licheniformis JCM 2505 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus licheniformis NRRL NRS-1264 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus velezensis BGSC 10A6 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus velezensis DSM 23117 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus amyloliquefaciens SMB469 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus licheniformis NBRC 12200 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus licheniformis ATCC 14580 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus velezensis FZB42 a peptide + a 5-L-glutamyl amino acid
-
 ?
a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus licheniformis DSM 13 a peptide + a 5-L-glutamyl amino acid
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycine very low activity Bacillus amyloliquefaciens 4-nitroaniline + 5-L-glutamyl-glycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycine very low activity Bacillus amyloliquefaciens SMB469 4-nitroaniline + 5-L-glutamyl-glycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
-
 Bacillus velezensis 4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycylglycine best substrate Bacillus licheniformis 4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycylglycine best substrate Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycylglycine best substrate Bacillus amyloliquefaciens 4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycylglycine best substrate Bacillus subtilis 168 4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycylglycine best substrate Bacillus licheniformis NCIMB 9375 4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycylglycine best substrate Bacillus licheniformis Gibson 46 4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycylglycine best substrate Bacillus licheniformis JCM 2505 4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycylglycine best substrate Bacillus licheniformis NRRL NRS-1264 4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
-
 Bacillus velezensis BGSC 10A6 4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
-
 Bacillus velezensis DSM 23117 4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycylglycine best substrate Bacillus amyloliquefaciens SMB469 4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycylglycine best substrate Bacillus licheniformis NBRC 12200 4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycylglycine best substrate Bacillus licheniformis ATCC 14580 4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
-
 Bacillus velezensis FZB42 4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + glycylglycine best substrate Bacillus licheniformis DSM 13 4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-arginine
-
 Bacillus licheniformis 4-nitroaniline + 5-L-glutamyl-L-arginine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-arginine
-
 Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-L-arginine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-arginine
-
 Bacillus amyloliquefaciens 4-nitroaniline + 5-L-glutamyl-L-arginine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-arginine
-
 Bacillus subtilis 168 4-nitroaniline + 5-L-glutamyl-L-arginine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-arginine
-
 Bacillus licheniformis NCIMB 9375 4-nitroaniline + 5-L-glutamyl-L-arginine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-arginine
-
 Bacillus licheniformis Gibson 46 4-nitroaniline + 5-L-glutamyl-L-arginine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-arginine
-
 Bacillus licheniformis JCM 2505 4-nitroaniline + 5-L-glutamyl-L-arginine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-arginine
-
 Bacillus licheniformis NRRL NRS-1264 4-nitroaniline + 5-L-glutamyl-L-arginine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-arginine
-
 Bacillus amyloliquefaciens SMB469 4-nitroaniline + 5-L-glutamyl-L-arginine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-arginine
-
 Bacillus licheniformis NBRC 12200 4-nitroaniline + 5-L-glutamyl-L-arginine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-arginine
-
 Bacillus licheniformis ATCC 14580 4-nitroaniline + 5-L-glutamyl-L-arginine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-arginine
-
 Bacillus licheniformis DSM 13 4-nitroaniline + 5-L-glutamyl-L-arginine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
-
 Bacillus licheniformis 4-nitroaniline + 5-L-glutamyl-L-asparagine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
-
 Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-L-asparagine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
-
 Bacillus amyloliquefaciens 4-nitroaniline + 5-L-glutamyl-L-asparagine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
-
 Bacillus subtilis 168 4-nitroaniline + 5-L-glutamyl-L-asparagine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
-
 Bacillus licheniformis NCIMB 9375 4-nitroaniline + 5-L-glutamyl-L-asparagine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
-
 Bacillus licheniformis Gibson 46 4-nitroaniline + 5-L-glutamyl-L-asparagine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
-
 Bacillus licheniformis JCM 2505 4-nitroaniline + 5-L-glutamyl-L-asparagine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
-
 Bacillus licheniformis NRRL NRS-1264 4-nitroaniline + 5-L-glutamyl-L-asparagine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
-
 Bacillus licheniformis NBRC 12200 4-nitroaniline + 5-L-glutamyl-L-asparagine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
-
 Bacillus licheniformis ATCC 14580 4-nitroaniline + 5-L-glutamyl-L-asparagine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
-
 Bacillus licheniformis DSM 13 4-nitroaniline + 5-L-glutamyl-L-asparagine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-histidine
-
 Bacillus licheniformis 4-nitroaniline + 5-L-glutamyl-L-histidine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-histidine
-
 Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-L-histidine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-histidine
-
 Bacillus amyloliquefaciens 4-nitroaniline + 5-L-glutamyl-L-histidine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-isoleucine
-
 Bacillus licheniformis 4-nitroaniline + 5-L-glutamyl-L-isoleucine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-isoleucine
-
 Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-L-isoleucine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-isoleucine
-
 Bacillus amyloliquefaciens 4-nitroaniline + 5-L-glutamyl-L-isoleucine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-leucine
-
 Bacillus licheniformis 4-nitroaniline + 5-L-glutamyl-L-leucine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-leucine
-
 Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-L-leucine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-leucine
-
 Bacillus amyloliquefaciens 4-nitroaniline + 5-L-glutamyl-L-leucine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-lysine
-
 Bacillus licheniformis 4-nitroaniline + 5-L-glutamyl-L-lysine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-lysine
-
 Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-L-lysine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-lysine
-
 Bacillus amyloliquefaciens 4-nitroaniline + 5-L-glutamyl-L-lysine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-methionine
-
 Bacillus licheniformis 4-nitroaniline + 5-L-glutamyl-L-methionine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-methionine
-
 Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-L-methionine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-methionine
-
 Bacillus amyloliquefaciens 4-nitroaniline + 5-L-glutamyl-L-methionine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-phenylalanine
-
 Bacillus licheniformis 4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-phenylalanine
-
 Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-phenylalanine
-
 Bacillus amyloliquefaciens 4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-proline very low activity Bacillus licheniformis 4-nitroaniline + 5-L-glutamyl-L-proline
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-threonine
-
 Bacillus licheniformis 4-nitroaniline + 5-L-glutamyl-L-threonine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-threonine
-
 Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-L-threonine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-threonine
-
 Bacillus amyloliquefaciens 4-nitroaniline + 5-L-glutamyl-L-threonine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-tryptophan
-
 Bacillus licheniformis 4-nitroaniline + 5-L-glutamyl-L-tryptophan
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-tryptophan
-
 Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-L-tryptophan
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-tryptophan
-
 Bacillus amyloliquefaciens 4-nitroaniline + 5-L-glutamyl-L-tryptophan
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-valine
-
 Bacillus licheniformis 4-nitroaniline + 5-L-glutamyl-L-valine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-valine
-
 Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-L-valine
-
 ?
L-gamma-glutamyl-4-nitroanilide + L-valine
-
 Bacillus amyloliquefaciens 4-nitroaniline + 5-L-glutamyl-L-valine
-
 ?
additional information substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with glycine Bacillus licheniformis ?
-
-
additional information substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with L-proline and glycine Bacillus subtilis ?
-
-
additional information substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with L-proline, poor activity with glycine Bacillus amyloliquefaciens ?
-
-
additional information substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with L-proline and glycine Bacillus subtilis 168 ?
-
-
additional information substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with glycine Bacillus licheniformis NCIMB 9375 ?
-
-
additional information substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with glycine Bacillus licheniformis Gibson 46 ?
-
-
additional information substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with glycine Bacillus licheniformis JCM 2505 ?
-
-
additional information substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with glycine Bacillus licheniformis NRRL NRS-1264 ?
-
-
additional information substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with L-proline, poor activity with glycine Bacillus amyloliquefaciens SMB469 ?
-
-
additional information substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with glycine Bacillus licheniformis NBRC 12200 ?
-
-
additional information substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with glycine Bacillus licheniformis ATCC 14580 ?
-
-
additional information substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with glycine Bacillus licheniformis DSM 13 ?
-
-

Subunits

Subunits Comment Organism
heterodimer
-
 Bacillus licheniformis
heterodimer
-
 Bacillus subtilis
heterodimer
-
 Bacillus velezensis
heterodimer 1 * 45700 + 1 * 19700, recombinant extracellular C-terminally His6-tagged enzyme, SDS-PAGE Bacillus amyloliquefaciens

Synonyms

Synonyms Comment Organism
BaGGT42
-
 Bacillus velezensis
BaGGT469
-
 Bacillus amyloliquefaciens
BlGGT13
-
 Bacillus licheniformis
BsGGT168
-
 Bacillus subtilis
gamma-glutamyltranspeptidase
-
 Bacillus licheniformis
gamma-glutamyltranspeptidase
-
 Bacillus subtilis
gamma-glutamyltranspeptidase
-
 Bacillus amyloliquefaciens
gamma-glutamyltranspeptidase
-
 Bacillus velezensis
GGT
-
 Bacillus licheniformis
GGT
-
 Bacillus subtilis
GGT
-
 Bacillus amyloliquefaciens
GGT
-
 Bacillus velezensis
More see also EC 3.4.19.13 Bacillus licheniformis
More see also EC 3.4.19.13 Bacillus subtilis
More see also EC 3.4.19.13 Bacillus amyloliquefaciens
More see also EC 3.4.19.13 Bacillus velezensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Bacillus licheniformis
40
-
 assay at Bacillus velezensis
45
-
-
 Bacillus subtilis
60
-
-
 Bacillus amyloliquefaciens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 activity range, 8.9% of maximal activity at 70°C, profile overview Bacillus amyloliquefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 thermal stability of BaGGT469 is extremely low and its residual activity is completely lost within 10 min at 60°C. But BaGGT469 is quite stable in the presence of substrate at 40°C Bacillus amyloliquefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8
-
 Bacillus licheniformis
9
-
 assay at Bacillus velezensis
9 9.5
-
 Bacillus subtilis
10
-
-
 Bacillus amyloliquefaciens

pH Range

pH Minimum pH Maximum Comment Organism
4 10 BaGGT469 shows the highest activity towards L-gamma-glutamyl-4-nitroanilide with diglycine acceptor in 50 mM borate-NaOH buffer at pH 10.0. The enzymatic activity at the alkaline pH range of pH 8.0-9.0 is 92.6-95.9% relative to that at optimum pH. BaGGT469 is much less active in the acidic pH range from pH 4.0-6.0 Bacillus amyloliquefaciens

General Information

General Information Comment Organism
evolution the deduced amino acid sequence of Bacillus amyloliquefaciens BaGGT469 is almost identical to that of Bacillus amyloliquefaciens BaGGT42 with the exception of only two amino acid residues (Val349Ile and Ser383Ala) Bacillus amyloliquefaciens
evolution the deduced amino acid sequence of Bacillus amyloliquefaciens BaGGT469 is almost identical to that of Bacillus amyloliquefaciens BaGGT42 with the exception of only two amino acid residues (Val349Ile and Ser383Ala) Bacillus velezensis
physiological function gamma-glutamyltranspeptidase (GGT) catalyzes the cleavage of gamma-glutamyl compounds and the transfer of gamma-glutamyl moiety to water or to amino acid/peptide acceptors Bacillus licheniformis
physiological function gamma-glutamyltranspeptidase (GGT) catalyzes the cleavage of gamma-glutamyl compounds and the transfer of gamma-glutamyl moiety to water or to amino acid/peptide acceptors Bacillus subtilis
physiological function gamma-glutamyltranspeptidase (GGT) catalyzes the cleavage of gamma-glutamyl compounds and the transfer of gamma-glutamyl moiety to water or to amino acid/peptide acceptors Bacillus amyloliquefaciens
physiological function gamma-glutamyltranspeptidase (GGT) catalyzes the cleavage of gamma-glutamyl compounds and the transfer of gamma-glutamyl moiety to water or to amino acid/peptide acceptors Bacillus velezensis