Any feedback?
Literature summary for 2.3.2.2 extracted from
- Synthesis of theanine from glutamic acid gamma-methyl ester and ethylamine catalyzed by Escherichia coli having gamma-glutamyltranspeptidase activity (2010), Biotechnol. Lett., 32, 1147-1150.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutamic acid gamma methyl ester + ethylamine | - |
Escherichia coli | theanine + ethanol | - |
ir |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gamma-glutamyltranspeptidase | - |
Escherichia coli |
| GGT | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
optimum for theanine synthesis | Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 55 | about 70% activity at 35°C, about 80% activity at 40°C, 100% activity at 45°C, about 85% activity at 50°C, about 45% activity at 55°C | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
optimum for theanine synthesis | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 11 | about 35% activity at pH 7.0, about 55% activity at pH 8.0, about 85% activity at pH 9.0, 100% acitivity at pH 10.0, about 75% activity at pH 11.0 | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
