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Literature summary for 2.3.2.2 extracted from

  • Wickham, S.; West, M.; Cook, P.; Hanigan, M.
    Gamma-glutamyl compounds: Substrate specificity of gamma-glutamyl transpeptidase enzymes (2011), Anal. Biochem., 414, 208-214.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
serine-borate competitive inhibitor which is 8fold more potent in inhibiting isoform GGT1 than in inhibiting isoform GGT5; competitive inhibitor which is 8fold more potent in inhibiting isoform GGT1 than in inhibiting isoform GGT5 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 GSSG isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
0.0099
-
 S-Methylglutathione isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
0.0102
-
 leukotriene C4 isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
0.0105
-
 GSH isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
0.0106
-
 GSH isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
0.0108
-
 leukotriene C4 isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
0.0131
-
 S-(4-nitro-benzyl)glutathione isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
0.0148
-
 S-(4-nitro-benzyl)glutathione isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
0.0182
-
 S-Methylglutathione isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
0.0334
-
 5-L-glutamyl-L-leucine isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
0.0346
-
 glutathione sulfonic acid isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
0.0426
-
 GSSG isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
0.0751
-
 glutathionesulfonic acid isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 1 * 64000 + 1 * 22000, isoform GGT1, SDS-PAGE Homo sapiens
25000
-
 1 * 64000 + 1 * 25000, isoform GGT5, SDS-PAGE Homo sapiens
64000
-
 1 * 64000 + 1 * 22000, isoform GGT1, SDS-PAGE Homo sapiens
64000
-
 1 * 64000 + 1 * 25000, isoform GGT5, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens P19440 isoform GGT1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-L-glutamyl-4-nitroanilide + glycylglycine substrate for isoform GGT1 Homo sapiens 5-L-glutamyl-glycylglycine + 4-nitroaniline
-
 ?
5-L-glutamyl-L-leucine + glycylglycine
-
 Homo sapiens L-Leu + 5-L-glutamyl-glycylglycine
-
 ?
glutathione sulfonic acid + glycylglycine
-
 Homo sapiens ?
-
 ?
glutathionesulfonic acid + glycylglycine
-
 Homo sapiens ?
-
 ?
GSH + glycylglycine
-
 Homo sapiens L-Cys-Gly + 5-L-glutamyl-glycylglycine
-
 ?
GSSG + glycylglycine
-
 Homo sapiens L-Cys-Gly + 5-L-glutamyl-glycylglycine
-
 ?
leukotriene C4 + glycylglycine
-
 Homo sapiens leukotriene D4 + 5-L-glutamyl-glycylglycine
-
 ?
additional information isoform GGT5 does not cleave 5-L-glutamyl-4-nitroanilide and gamma-glutamyl leucine Homo sapiens ?
-
 ?
S-(4-nitro-benzyl)glutathione + glycylglycine
-
 Homo sapiens ?
-
 ?
S-methylglutathione + glycylglycine
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 64000 + 1 * 22000, isoform GGT1, SDS-PAGE Homo sapiens
heterodimer 1 * 64000 + 1 * 25000, isoform GGT5, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
gamma-glutamyl transpeptidase
-
 Homo sapiens
GGT1 isoform Homo sapiens
GGT5 isoform Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2
-
 serine-borate isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens
41.6
-
 serine-borate isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C Homo sapiens