Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus licheniformis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of full length gene and six truncations lacking 36, 129, 132, 135, 144, and 174 bp, respectively, at the 5' end in Escherichia coli. Mutant proteins derived from genes lacking 135 and 144 bp show no enzymatic activity. Mutants derived of truncations of 36. 129, 132 process autocatalytically their precursors into alpha- und beta-subunits at 4°C | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2 | - |
mutant derived from truncation of 132 bp at the 5' end, pH 8.0, 40°C | Bacillus licheniformis |
0.8 | - |
mutant derived from truncation of 129 bp at the 5' end, pH 8.0, 40°C | Bacillus licheniformis |
1.3 | - |
mutant derived from truncation of 36 bp at the 5' end, pH 8.0, 40°C | Bacillus licheniformis |
51.9 | - |
wild-type, pH 8.0, 40°C | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-L-glutamyl-4-nitroanilide + Gly-Gly | - |
Bacillus licheniformis | 4-nitroaniline + 5-L-glutamyl-Gly-Gly | - |
? |