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Literature summary for 2.3.2.2 extracted from

  • Nakayama, R.; Kumagai, H.; Tochikura, T.
    Purification and properties of gamma-glutamyltranspeptidase from Proteus mirabilis (1984), J. Bacteriol., 160, 341-346.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.2

Crystallization (Commentary)

Crystallization (Comment) Organism
ammonium sulfate precipitation, refrigerator, 2 weeks Proteus mirabilis

Inhibitors

Inhibitors Comment Organism Structure
6-diazo-5-oxo-L-norleucine
-
 Proteus mirabilis
Aminooxyacetate hydrolase reaction Proteus mirabilis
beta-chloro-L-alanine
-
 Proteus mirabilis
glutathione no inhibition Proteus mirabilis
glycine weak Proteus mirabilis
Hg2+
-
 Proteus mirabilis
iodoacetamide
-
 Proteus mirabilis
iodoacetate weak Proteus mirabilis
L- or D-Serine/borate L-serine/borate, not D-serine/borate Proteus mirabilis
L-alanine
-
 Proteus mirabilis
L-glutamine not D- Proteus mirabilis
additional information inhibition by diverse amino acids, overview Proteus mirabilis
N-ethylmaleimide no inhibition Proteus mirabilis
p-chloromercuribenzoate no inhibition Proteus mirabilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
 glutathione hydrolase reaction Proteus mirabilis
0.13 0.18 L-Glu-4-nitroanilide with GSH Proteus mirabilis
0.18
-
 glutathione transpeptidation Proteus mirabilis
0.31
-
 L-Glu-4-nitroanilide hydrolase reaction Proteus mirabilis
0.4
-
 L-Glu-4-nitroanilide transpeptidation Proteus mirabilis
2.9 2.96 glycylglycine
-
 Proteus mirabilis
10
-
 L-glutamine hydrolase reaction Proteus mirabilis
32
-
 L-Phe
-
 Proteus mirabilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 1 * 47000 + 1 * 28000, SDS-PAGE Proteus mirabilis
47000
-
 1 * 47000 + 1 * 28000, SDS-PAGE Proteus mirabilis
80000
-
 gel filtration Proteus mirabilis

Organism

Organism UniProt Comment Textmining
Proteus mirabilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Proteus mirabilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16
-
 purified enzyme Proteus mirabilis

Storage Stability

Storage Stability Organism
4°C, crystalline, at least 3 months Proteus mirabilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: hydrolase reaction with H2O as acceptor Proteus mirabilis peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ acceptors are amino acids or dipeptides Proteus mirabilis peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: autotranspeptidation with another donor molecule as acceptor Proteus mirabilis peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Proteus mirabilis peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ donor specificity, overview Proteus mirabilis peptide + 5-L-glutamyl amino acid
-
 ?
5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Proteus mirabilis 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
GSH + L-glutamine
-
 Proteus mirabilis ?
-
 ?
GSH + L-Phe
-
 Proteus mirabilis ?
-
 ?
L-Glu-4-nitroanilide + glutathione
-
 Proteus mirabilis 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 47000 + 1 * 28000, SDS-PAGE Proteus mirabilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Proteus mirabilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 10% loss of activity within 10 min Proteus mirabilis
45
-
 37% loss of activity within 10 min Proteus mirabilis
50
-
 95% loss of activity within 10 min Proteus mirabilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 hydrolase reaction Proteus mirabilis
7.5 8 transpeptidation Proteus mirabilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 50% loss of activity after 60 min, 37°C Proteus mirabilis
5 9 at least 60 min stable, 37°C Proteus mirabilis
10
-
 50% loss of activity after 60 min, 37°C Proteus mirabilis