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Literature summary for 2.3.2.2 extracted from

  • Suzuki, H.; Kumagai, H.; Tochikura, T.
    gamma-Glutamyltranspeptidase from Escherichia coli K-12: purification and properties (1986), J. Bacteriol., 168, 1325-1331.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.2

Crystallization (Commentary)

Crystallization (Comment) Organism
ammonium sulfate precipitation from 20 mM Tris-HCl, pH 8.0, 14.2 mg/ml protein, refrigerator, 1 week Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
1-chloro-3-tosylamido-7-amino-2-heptanone weak Escherichia coli
6-diazo-5-oxo-L-norleucine
-
 Escherichia coli
Hg2+ hydrolase, not transferase reaction Escherichia coli
iodoacetate weak Escherichia coli
L- or D-Serine/borate L-serine/borate, not D-serine/borate Escherichia coli
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid i.e. AT-125; strong, irreversible, 1 mM Escherichia coli
L-1-tosylamido-2-phenylethylchloromethylketone
-
 Escherichia coli
L-alanine
-
 Escherichia coli
L-azaserine
-
 Escherichia coli
L-glutamine not D- Escherichia coli
additional information
-
 Escherichia coli
N-ethylmaleimide weak Escherichia coli
p-chloromercuribenzoate no inhibition Escherichia coli
PMSF weak Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029 0.035 L-cystine hydrolase reaction Escherichia coli
0.029 0.035 L-cystine glutathione Escherichia coli
0.035
-
 glutathione
-
 Escherichia coli
0.035
-
 L-Glu-4-nitroanilide
-
 Escherichia coli
0.068
-
 L-Glu-4-nitroanilide hydrolase reaction Escherichia coli
210
-
 L-Arg
-
 Escherichia coli
590
-
 glycylglycine
-
 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Escherichia coli
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 1 * 39200 + 1 * 22000, isoform A, SDS-PAGE Escherichia coli
22000
-
 1 * 38600 + 1 * 22000, isoform B, SDS-PAGE Escherichia coli
38600
-
 1 * 38600 + 1 * 22000, isoform B, SDS-PAGE Escherichia coli
39200
-
 1 * 39200 + 1 * 22000, isoform A, SDS-PAGE Escherichia coli
57000
-
 isoform B, gel filtration Escherichia coli
58000
-
 isoform A, gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 K-12, strain MG1655
-
Escherichia coli
-
 2 isozymes A and B
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4
-
 purified isozymes A and B Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: hydrolase reaction with H2O as acceptor Escherichia coli peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is 5-L-Glu-4-nitroanilide Escherichia coli peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ acceptors are amino acids or dipeptides Escherichia coli peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ isozyme A prefers basic and aromatic amino acids as acceptors Escherichia coli peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: autotranspeptidation with another donor molecule as acceptor Escherichia coli peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is GSH Escherichia coli peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ donor specificity, overview Escherichia coli peptide + 5-L-glutamyl amino acid
-
 ?
5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Escherichia coli 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
5-L-glutamyl-4-nitroanilide + L-asparagine
-
 Escherichia coli 4-nitroaniline + 5-L-glutamyl-L-asparagine
-
 ?
5-L-glutamyl-4-nitroanilide + L-cystine
-
 Escherichia coli 4-nitroaniline + 5-L-glutamyl-L-cystine
-
 ?
glutathione + acceptor acceptor: amino acids Escherichia coli cysteinylglycine + 5-L-glutamyl-acceptor
-
 ?
GSH + L-Arg
-
 Escherichia coli ?
-
 ?
L-Glu-4-nitroanilide + glutathione
-
 Escherichia coli 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 39200 + 1 * 22000, isoform A, SDS-PAGE Escherichia coli
dimer 1 * 38600 + 1 * 22000, isoform B, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 12% loss of activity within 15 min Escherichia coli
48
-
 t1/2: 15 min Escherichia coli
50
-
 92% loss of activity within 15 min Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
 transpeptidation Escherichia coli
9.5
-
 hydrolase reaction Escherichia coli