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Literature summary for 2.3.2.2 extracted from
- Studies of human kidney gamma-glutamyl transpeptidase. Purification and structural, kinetic and immunological properties (1976), J. Biol. Chem., 251, 2271-2278.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | effect of amino acids on activity | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | weak | Homo sapiens |  |
| glutathione | - |
Homo sapiens | |
| iodoacetate | weak | Homo sapiens | |
| Maleate | - |
Homo sapiens | |
| additional information | inhibition by diverse amino acids, overview | Homo sapiens | |
| N-ethylmaleimide | - |
Homo sapiens | |
| NH4+ | weak | Homo sapiens | |
| p-hydroxymercuribenzoate | weak | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
L-Glu-4-nitroanilide | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | no effect | Homo sapiens | |
| K+ | no effect | Homo sapiens | |
| Li+ | no effect | Homo sapiens | |
| Mg2+ | no effect | Homo sapiens | |
| additional information | no activation by Zn2+ and Mn2+ | Homo sapiens | |
| Na+ | no effect | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Homo sapiens |
| additional information | - |
in the presence of Triton X-100: molecular aggregation | Homo sapiens |
| 21000 | - |
1 * 57000 + 1 * 21000, SDS-PAGE, presence of urea, 2-mercaptoethanol | Homo sapiens |
| 57000 | - |
1 * 57000 + 1 * 21000, SDS-PAGE, presence of urea, 2-mercaptoethanol | Homo sapiens |
| 90000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 200.4 | - |
purified enzyme | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, t1/2: 2 months | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (5-L-glutamyl)-peptide + acceptor + H+ | - |
Homo sapiens | peptide + 5-L-glutamyl amino acid | - |
? | |
| 5-L-glutamyl-4-nitroanilide + glycylglycine | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamylglycylglycine | - |
? | |
| 5-L-glutamyl-4-nitroanilide + L-glutamine | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamyl-L-glutamine | - |
? | |
| glutathione + H2O | hydrolase reaction, concurrent to (auto-)transpeptidation | Homo sapiens | L-cysteinylglycine + L-glutamate | - |
? | |
| L-Glu-4-nitroanilide + glutathione | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamyl-glutathione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 57000 + 1 * 21000, SDS-PAGE, presence of urea, 2-mercaptoethanol | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 4.5 | Homo sapiens |
| 8 | - |
assay at | Homo sapiens |
| 8.2 | - |
- |
Homo sapiens |
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