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Literature summary for 2.3.2.2 extracted from

  • Ding, J.L.; Smith, G.D.; Peters, T.J.
    Purification and properties of gamma-glutamyl transferase from normal rat liver (1981), Biochim. Biophys. Acta, 657, 334-343.
    View publication on PubMed
Search for more literature for 2.3.2.2

Inhibitors

Inhibitors Comment Organism Structure
EDTA no inhibition Rattus norvegicus
Mg2+ no inhibition Rattus norvegicus
p-chloromercuribenzoate weak Rattus norvegicus
Zn2+ 50% inhibition at 0.4 mM; reversible by EDTA Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.39
-
 5-L-glutamyl-7-amido-4-methylcoumarin liver enzyme Rattus norvegicus
0.49
-
 5-L-glutamyl-7-amido-4-methylcoumarin biliary tract enzyme Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ no effect Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 in the presence of Triton X-100: molecular aggregation Rattus norvegicus
250000
-
 gel filtration in the presence of Triton X-100 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 male, Sprague-Dawley
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein binding of concanavalin A Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
partial from liver, highly from biliary tract Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
bile duct 80-90% of activity Rattus norvegicus
-
liver 80-90% of activity in biliary tract, distribution in liver tissues Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
218
-
 partially purified enzyme from liver Rattus norvegicus
398
-
 purified enzyme from biliary tract Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+
-
 Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine
-
 Rattus norvegicus 7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus