Application | Comment | Organism |
---|---|---|
biotechnology | enhanced biosynthesis of cadmium(II)-selenide (CdS) nanoparticles (NPs) through Arabidopsis thaliana phytochelatin synthase-modified Escherichia coli with fluorescence effect in detection of pyrogallol and gallic acid. CdS NPs act as a classical II-VI semiconductor with various interesting properties and incomparable advantages. Enzyme PCS can effectively capture and enrich Cd2+ so that the generation and subsequent application process of CdS NPs will be greatly improved. A direct detection method based on such CdS NPs is established for determination of pyrogallol and gallic acid, two organic molecules with multiple advantages and wide applications. The method provides an excellent alternative to traditional chemosynthesis and quantitative analysis, which also displays great potential for technological improvement towards green synthesis and application. Method development and evaluation, detailed overview | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
gene PCS1, recombinant expression in Escherichia coli strain Rosetta (DE3) pLysS | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + [Glu(-Cys)]n-Gly | Arabidopsis thaliana | - |
Gly + [Glu(-Cys)]n+1-Gly | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9S7Z3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + [Glu(-Cys)]n-Gly | - |
Arabidopsis thaliana | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
additional information | the PCS enzyme catalyzes transformation of GSH to give phytochelatins (PCs), it can effectively capture and enrich Cd2+ so that the generation and subsequent application process of CdS NPs is greatly improved | Arabidopsis thaliana | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 54500, recombinant enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AtPCS1 | - |
Arabidopsis thaliana |
PCS | - |
Arabidopsis thaliana |
PCS1 | - |
Arabidopsis thaliana |
phytochelatin synthase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | activity range | Arabidopsis thaliana |