Cloned (Comment) | Organism |
---|---|
gene PCS1, two Morus notabilis PCS genes are identified based on a genome-wide analysis of the Morus genome database, DNA and amino acid sequence determination and analysis, exon-intron structures of the MnPCS genes, quantitative real-time PCR enzyme expression analysis, expression in Arabidopsis thaliana | Morus notabilis |
gene PCS2, two Morus notabilis PCS genes are identified based on a genome-wide analysis of the Morus genome database, DNA and amino acid sequence determination and analysis, exon-intron structures of the MnPCS genes, quantitative real-time PCR enzyme expression analysis, recombinant expression in Arabidopsis thaliana | Morus notabilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Zn2+/Cd2+ concentrations in both shoots and roots of the transgenic Arabidopsis seedlings expressing PCS1 and/or PCS2 are higher than in wild-type seedlings at two different Zn2+/Cd2+ concentrations. In addition, there is a positive correlation between Zn2+ accumulation and the expression level of MnPCS1 or MnPCS2 | Morus notabilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Morus notabilis | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + [Glu(-Cys)]n-Gly | Morus notabilis | - |
Gly + [Glu(-Cys)]n+1-Gly | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Morus notabilis | W9QSJ2 | - |
- |
Morus notabilis | W9QSW3 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Morus notabilis | - |
seedling | - |
Morus notabilis | - |
shoot | - |
Morus notabilis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + [Glu(-Cys)]n-Gly | - |
Morus notabilis | Gly + [Glu(-Cys)]n+1-Gly | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MnPCS1 | - |
Morus notabilis |
MnPCS2 | - |
Morus notabilis |
PCS | - |
Morus notabilis |
PCS1 | - |
Morus notabilis |
PCS2 | - |
Morus notabilis |
phytochelatin synthase | - |
Morus notabilis |
Organism | Comment | Expression |
---|---|---|
Morus notabilis | expression of PCS1 is induced in root, stem, and leaf tissues within 24 h of exposure to 0.3 mM Zn2+ and 0.03-0.1 mM Cd2+ | up |
Morus notabilis | expression of PCS2 is induced in root, stem, and leaf tissues within 24 h of exposure to 0.3 mM Zn2+ and 0.03-0.1 mM Cd2+ | up |
General Information | Comment | Organism |
---|---|---|
physiological function | phytochelatin synthase (PCS) is an enzyme involved in the synthesis of phytochelatins, cysteine-rich peptides which play a key role in heavy metal (HM) detoxification of plants. Mulberry has the potential to remediate HM-contaminated soils. The two PCS genes in Morus notabilis (PCS1 and 2) are involved in HM detoxification in Morus. MnPCS1 plays a more important role in Cd detoxification than MnPCS2. MnPCS enzymes can markedly increase the tolerance of the transgenic plants at high concentrations of Zn2+, while the corresponding relationship between Cd2+ accumulation and the expression of MnPCSs are not observed. There might be some factor-dependent posttranscriptional regulation of PCS, like intron-mediated enhancement, and/or an optimum PCS level for tolerance to and accumulation of HMs in plants | Morus notabilis |
physiological function | phytochelatin synthase (PCS) is an enzyme involved in the synthesis of phytochelatins, cysteine-rich peptides which play a key role in heavy metal (HM) detoxification of plants. Mulberry has the potential to remediate HM-contaminated soils. The two PCS genes in Morus notabilis (PCS1 and 2) are involved in HM detoxification in Morus. MnPCS1 plays a more important role in Cd detoxification than MnPCS2. The enzymes can markedly increase the tolerance of the transgenic plants at high concentrations of Zn2+, while the corresponding relationship between Cd2+ accumulation and the expression of MnPCSs are not observed. There might be some factor-dependent posttranscriptional regulation of PCS, like intron-mediated enhancement, and/or an optimum PCS level for tolerance to and accumulation of HMs in plants | Morus notabilis |