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Literature summary for 2.3.2.15 extracted from

  • Cahoon, R.E.; Lutke, W.K.; Cameron, J.C.; Chen, S.; Lee, S.G.; Rivard, R.S.; Rea, P.A.; Jez, J.M.
    Adaptive engineering of phytochelatin-based heavy metal tolerance (2015), J. Biol. Chem., 290, 17321-17330.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells and Saccharomyces cerevisiae strain TY167 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
A182G/A282V/G329S the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
A59V the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
C109Y the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
C91S/A199S the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
D71N the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
E52K the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
F83C/N170D the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
Q48R/C144Y/G168S/W280R the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
R74H/S230C/L250R the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
S51T/N143I/N170I/H220R the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
S60C/S202I the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
T123R/F163I the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
T139P the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
V181G the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
V97C the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
V97L the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
Y186C the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, FLAG-tagged enzyme, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione + [Glu(-Cys)]n-Gly Arabidopsis thaliana
-
 Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9S7Z3
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3
-
 mutant enzyme Y186C, at pH 8.0 and 30°C Arabidopsis thaliana
1.8
-
 mutant enzyme C109Y, at pH 8.0 and 30°C Arabidopsis thaliana
2.6
-
 mutant enzyme T139P, at pH 8.0 and 30°C Arabidopsis thaliana
2.8
-
 mutant enzyme V181G, at pH 8.0 and 30°C Arabidopsis thaliana
2.8
-
 mutant enzyme V97C, at pH 8.0 and 30°C Arabidopsis thaliana
2.9
-
 mutant enzyme A59V, at pH 8.0 and 30°C Arabidopsis thaliana
3.1
-
 mutant enzyme F83C/N170D, at pH 8.0 and 30°C Arabidopsis thaliana
3.2
-
 mutant enzyme E52K, at pH 8.0 and 30°C Arabidopsis thaliana
3.3
-
 mutant enzyme S51T/N143I/N170I/H220R, at pH 8.0 and 30°C Arabidopsis thaliana
3.6
-
 mutant enzyme T123R/F163I, at pH 8.0 and 30°C Arabidopsis thaliana
3.7
-
 mutant enzyme R74H/S230C/L250R, at pH 8.0 and 30°C Arabidopsis thaliana
4.6
-
 mutant enzyme C91S/A199S, at pH 8.0 and 30°C Arabidopsis thaliana
4.8
-
 mutant enzyme V97L, at pH 8.0 and 30°C Arabidopsis thaliana
5
-
 mutant enzyme Q48R/C144Y/G168S/W280R, at pH 8.0 and 30°C Arabidopsis thaliana
5.1
-
 mutant enzyme D71N, at pH 8.0 and 30°C Arabidopsis thaliana
5.5
-
 mutant enzyme A182G/A282V/G329S, at pH 8.0 and 30°C Arabidopsis thaliana
5.9
-
 mutant enzyme S60C/S202I, at pH 8.0 and 30°C Arabidopsis thaliana
16.8
-
 wild type enzyme, at pH 8.0 and 30°C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + [Glu(-Cys)]n-Gly
-
 Arabidopsis thaliana Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, FLAG-tagged enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
PCS1
-
 Arabidopsis thaliana
phytochelatin synthase
-
 Arabidopsis thaliana