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Literature summary for 2.3.2.15 extracted from

  • Chang, Y.; Li, H.; Cong, Y.; Lin, J.; Sheng, B.
    Characterization and Expression of a phytochelatin synthase gene in birch-leaf pear (Pyrus betulaefolia Bunge) (2012), Plant Mol. Biol. Rep., 30, 1329-1337.
No PubMed abstract available
Search for more literature for 2.3.2.15

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pyrus betulifolia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, calculated from amino acid sequence Pyrus betulifolia

Organism

Organism UniProt Comment Textmining
Pyrus betulifolia H2DFA2 isoform PCS1; birch-leaf pear
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Pyrus betulifolia
-
root isoform PCS1 expression levels are higher in roots than in leaves and stems Pyrus betulifolia
-
seedling isoform PCS1 has a very low basal expression level in untreated whole seedlings Pyrus betulifolia
-
stem
-
 Pyrus betulifolia
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + [Glu(-Cys)]n-Gly
-
 Pyrus betulifolia Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, calculated from amino acid sequence Pyrus betulifolia

Synonyms

Synonyms Comment Organism
gamma-glutamylcysteine transpeptidase
-
 Pyrus betulifolia
PCS1
-
 Pyrus betulifolia
phytochelatin synthase
-
 Pyrus betulifolia

pI Value

Organism Comment pI Value Maximum pI Value
Pyrus betulifolia calculated from amino acid sequence
-
 9.4

Expression

Organism Comment Expression
Pyrus betulifolia L-buthionine-sulfoximine can inhibit isoform PCS1 expression in roots, stems and leaves down
Pyrus betulifolia after 24 h of exposure to 0.02 mM CdSO4, CuSO4 or ZnSO4, isoform PCS1 expression increases significantly. The increases in roots are in order of 4.34fold, 0.26fold or 2.82fold after exposure to CdSO4, CuSO4 or ZnSO4, respectively. Reduced L-glutathione stimulates isoform PCS1 expression in roots, stems and leaves (1.45-2.85fold) up