Application | Comment | Organism |
---|---|---|
analysis | a HPLC method for the analysis of the activity of phytochelatin synthase is developed | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
into the vector pET-28b for expression in Escherichia coli BL21 Rosetta DE3 pLysS cells | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56500 | - |
determined by SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + [Glu(-Cys)]n-Gly | Arabidopsis thaliana | - |
Gly + [Glu(-Cys)]n+1-Gly | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9S7Z3 | - |
- |
Purification (Comment) | Organism |
---|---|
on a Ni2+ affinity column | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.09 | - |
10 ng/ml rAtPCS1 | Arabidopsis thaliana |
6.6 | - |
30 ng/ml rAtPCS1 | Arabidopsis thaliana |
12.6 | - |
50 ng/ml rAtPCS1 | Arabidopsis thaliana |
18.4 | - |
10 ng/ml rAtPCS1, 100 ng/ml bovine serum albumin, BSA | Arabidopsis thaliana |
20.9 | - |
1 ng/ml rAtPCS1, 10000 ng/ml bovine serum albumin, BSA | Arabidopsis thaliana |
24.9 | - |
100 ng/ml rAtPCS1 | Arabidopsis thaliana |
25.9 | - |
200 ng/ml rAtPCS1 | Arabidopsis thaliana |
30 | - |
10 ng/ml rAtPCS1, 10000 ng/ml bovine serum albumin, BSA | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + [Glu(-Cys)]n-Gly | - |
Arabidopsis thaliana | Gly + [Glu(-Cys)]n+1-Gly | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtPCS1 | - |
Arabidopsis thaliana |
PCS1 | - |
Arabidopsis thaliana |
phytochelatin synthase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
activity assay | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Arabidopsis thaliana |