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Literature summary for 2.3.2.15 extracted from

  • Wang, H.; Wu, J.; Chia, J.; Yang, C.; Wu, Y.; Juang, R.
    Phytochelatin synthase is regulated by protein phosphorylation at a threonine residue near its catalytic site (2009), J. Agric. Food Chem., 57, 7348-7355.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Cd2+ the activity of PCS requires Cd and is enhanced by protein phosphorylation Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
the coding sequences of full-length PCS1, PCS-N, residues 1-221, and PCS-C, residues 222-485, are cloned into the vector pGEM-T-Easy and subsequently into pET28b for expression in Escherichia coli BL21DE3 cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
R183a AtPCS1 mutant, Arg183 is critical to the activity of PCS Arabidopsis thaliana
R183a AtPCS1-N mutant, Arg183 is critical to the activity of PCS Arabidopsis thaliana
T49A AtPCS1 mutant, Thr49 is the only residue to be phosphorylated Arabidopsis thaliana
T49A AtPCS1-N mutant, Thr49 is the only residue to be phosphorylated Arabidopsis thaliana
Y55A AtPCS1 mutant Arabidopsis thaliana
Y55A AtPCS1-N mutant Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26700
-
AtPCS1-N, residues 1-221, theoretical, verified by SDS-PAGE and Western Blot analysis Arabidopsis thaliana
31400
-
AtPCS1-C, residues 222-485, theoretical, verified by SDS-PAGE and Western Blot analysis Arabidopsis thaliana
56300
-
AtPCS1, residues 1-485, theoretical, verified by SDS-PAGE and Western Blot analysis Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione + [Glu(-Cys)]n-Gly Arabidopsis thaliana
-
Gly + [Glu(-Cys)]n+1-Gly
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9S7Z3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein PCS1 expressed in Escherichia coli is phosphorylated at a Thr residue near its catalytic site Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
using a HisTrap FF column Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + [Glu(-Cys)]n-Gly
-
Arabidopsis thaliana Gly + [Glu(-Cys)]n+1-Gly
-
?

Synonyms

Synonyms Comment Organism
AtPCS1
-
Arabidopsis thaliana
gamma-glutamyl-cysteine transpeptidase
-
Arabidopsis thaliana
PCS
-
Arabidopsis thaliana
PCS1
-
Arabidopsis thaliana
phytochelatin synthase
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
activity assay Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
activity assay Arabidopsis thaliana