Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | the activity of PCS requires Cd and is enhanced by protein phosphorylation | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
the coding sequences of full-length PCS1, PCS-N, residues 1-221, and PCS-C, residues 222-485, are cloned into the vector pGEM-T-Easy and subsequently into pET28b for expression in Escherichia coli BL21DE3 cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
R183a | AtPCS1 mutant, Arg183 is critical to the activity of PCS | Arabidopsis thaliana |
R183a | AtPCS1-N mutant, Arg183 is critical to the activity of PCS | Arabidopsis thaliana |
T49A | AtPCS1 mutant, Thr49 is the only residue to be phosphorylated | Arabidopsis thaliana |
T49A | AtPCS1-N mutant, Thr49 is the only residue to be phosphorylated | Arabidopsis thaliana |
Y55A | AtPCS1 mutant | Arabidopsis thaliana |
Y55A | AtPCS1-N mutant | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26700 | - |
AtPCS1-N, residues 1-221, theoretical, verified by SDS-PAGE and Western Blot analysis | Arabidopsis thaliana |
31400 | - |
AtPCS1-C, residues 222-485, theoretical, verified by SDS-PAGE and Western Blot analysis | Arabidopsis thaliana |
56300 | - |
AtPCS1, residues 1-485, theoretical, verified by SDS-PAGE and Western Blot analysis | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + [Glu(-Cys)]n-Gly | Arabidopsis thaliana | - |
Gly + [Glu(-Cys)]n+1-Gly | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9S7Z3 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | PCS1 expressed in Escherichia coli is phosphorylated at a Thr residue near its catalytic site | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
using a HisTrap FF column | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + [Glu(-Cys)]n-Gly | - |
Arabidopsis thaliana | Gly + [Glu(-Cys)]n+1-Gly | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtPCS1 | - |
Arabidopsis thaliana |
gamma-glutamyl-cysteine transpeptidase | - |
Arabidopsis thaliana |
PCS | - |
Arabidopsis thaliana |
PCS1 | - |
Arabidopsis thaliana |
phytochelatin synthase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
activity assay | Arabidopsis thaliana |