Cloned (Comment) | Organism |
---|---|
- |
Nostoc sp. |
- |
Arabidopsis thaliana |
expressed in Escherichia coli BL21(DE3) cells | Nostoc sp. |
expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
DELTA222-485 | truncation mutant is fulla sufficient for phytochelatin synthesis. The fragment may be insufficient to maintain the active form of the enzyme stably in vitro | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | required, 0.5 mM | Nostoc sp. | |
Cd2+ | required, 0.5 mM | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9S7Z3 | - |
- |
Nostoc sp. | - |
- |
- |
Nostoc sp. | - |
PCC7120 | - |
Nostoc sp. PCC 7120 | - |
PCC7120 | - |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl column chromatography and HiTrap SP column chromatography | Nostoc sp. |
DEAE-Toyopearl column chromatography, HiTrap SP column chromatography, and Hitrap Q sepharose column chromatography | Arabidopsis thaliana |
recombinant enzyme | Nostoc sp. |
recombinant enzyme | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + [Glu(-Cys)]n-Gly | - |
Nostoc sp. | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
glutathione + [Glu(-Cys)]n-Gly | - |
Arabidopsis thaliana | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
glutathione + [Glu(-Cys)]n-Gly | - |
Nostoc sp. PCC 7120 | Gly + [Glu(-Cys)]n+1-Gly | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtPCS | - |
Arabidopsis thaliana |
NsPCS | - |
Nostoc sp. |
PC synthase | - |
Nostoc sp. |
PC synthase | - |
Arabidopsis thaliana |
PCS | - |
Nostoc sp. |
PCS1 | - |
Arabidopsis thaliana |
phytochelatin synthase | - |
Nostoc sp. |
phytochelatin synthase | - |
Arabidopsis thaliana |