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Literature summary for 2.3.2.15 extracted from

  • Tsuji, N.; Nishikori, S.; Iwabe, O.; Matsumoto, S.; Shiraki, K.; Miyasaka, H.; Takagi, M.; Miyamoto, K.; Hirata, K.
    Comparative analysis of the two-step reaction catalyzed by prokaryotic and eukaryotic phytochelatin synthase by an ion-pair liquid chromatography assay (2005), Planta, 222, 181-191.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Nostoc sp.
-
Arabidopsis thaliana
expressed in Escherichia coli BL21(DE3) cells Nostoc sp.
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
DELTA222-485 truncation mutant is fulla sufficient for phytochelatin synthesis. The fragment may be insufficient to maintain the active form of the enzyme stably in vitro Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ required, 0.5 mM Nostoc sp.
Cd2+ required, 0.5 mM Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9S7Z3
-
-
Nostoc sp.
-
-
-
Nostoc sp.
-
PCC7120
-
Nostoc sp. PCC 7120
-
PCC7120
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Toyopearl column chromatography and HiTrap SP column chromatography Nostoc sp.
DEAE-Toyopearl column chromatography, HiTrap SP column chromatography, and Hitrap Q sepharose column chromatography Arabidopsis thaliana
recombinant enzyme Nostoc sp.
recombinant enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + [Glu(-Cys)]n-Gly
-
Nostoc sp. Gly + [Glu(-Cys)]n+1-Gly
-
?
glutathione + [Glu(-Cys)]n-Gly
-
Arabidopsis thaliana Gly + [Glu(-Cys)]n+1-Gly
-
?
glutathione + [Glu(-Cys)]n-Gly
-
Nostoc sp. PCC 7120 Gly + [Glu(-Cys)]n+1-Gly
-
?

Synonyms

Synonyms Comment Organism
AtPCS
-
Arabidopsis thaliana
NsPCS
-
Nostoc sp.
PC synthase
-
Nostoc sp.
PC synthase
-
Arabidopsis thaliana
PCS
-
Nostoc sp.
PCS1
-
Arabidopsis thaliana
phytochelatin synthase
-
Nostoc sp.
phytochelatin synthase
-
Arabidopsis thaliana