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Literature summary for 2.3.2.15 extracted from

  • Chen, J.; Zhou, J.; Goldsbrough, P.B.
    Characterization of phytochelatin synthase from tomato (1997), Physiol. Plant., 101, 165-172.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.7
-
glutathione
-
Solanum lycopersicum

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ activation Solanum lycopersicum
Au+ activation Solanum lycopersicum
Cd2+ activation Solanum lycopersicum
Cd2+ best at 0.5 mM Solanum lycopersicum
Cu2+ activation Solanum lycopersicum
Fe2+ activation Solanum lycopersicum
Hg2+ activation Solanum lycopersicum
Pb2+ activation Solanum lycopersicum
Zn2+ activation Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
tomato, strain Mill. cv. VFNT-Cherry
-

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Solanum lycopersicum
-
stem
-
Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0055
-
-
Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + [Glu(-Cys)]n-Gly
-
Solanum lycopersicum Gly + [Glu(-Cys)]n+1-Gly
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
half maximal activity at pH 6.2 and 9.2 Solanum lycopersicum