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Literature summary for 2.3.2.15 extracted from

  • Nakazawa, R.; Kato, H.; Kameda, Y.; Takenaga, H.
    Optimum assay conditions of the activity of phytochelatin synthase from tobacco cells (2002), Biol. Plant., 45, 311-313.
No PubMed abstract available
Search for more literature for 2.3.2.15

General Stability

General Stability Organism
at 30% glycerol,m/v, stability enhancement Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.6
-
 glutathione
-
 Nicotiana tabacum

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ activation Nicotiana tabacum
Cd2+ activation Nicotiana tabacum
Cd2+ best at 0.05 mM Nicotiana tabacum
Cu2+ activation Nicotiana tabacum
Hg2+ activation Nicotiana tabacum
Pb2+ activation Nicotiana tabacum
Zn2+ activation Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
 cv. Bright Yellow-2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + [Glu(-Cys)]n-Gly
-
 Nicotiana tabacum Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Nicotiana tabacum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 50% activity after 6 h Nicotiana tabacum
35
-
 50% activity after 0.5 h Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Nicotiana tabacum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
 most stable Nicotiana tabacum