Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | irreversible inhibition | Cavia porcellus | |
N-[(5S)-6-[4-(6-methylpyridin-2-yl)piperazin-1-yl]-6-oxo-5-(2-phenylacetamido)hexyl]prop-2-enamide | an selective Nepsilon-acryloyllysine derivative, irreversible inhibition | Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, kinetic analysis of TGase 2-catalysed conversions of the glutamate-derived acyl donors, detailed overview | Cavia porcellus | |
0.00114 | - |
Z-Phe-gamma-Abu-7-hydroxy-4-methylcoumarin | pH 8.0, 30°C | Cavia porcellus | |
0.00147 | - |
Z-Phe-gamma-Abu-7-hydroxycoumarin | pH 8.0, 30°C | Cavia porcellus | |
0.00253 | - |
Z-Glu(HC)-Gly-OH | pH 8.0, 30°C | Cavia porcellus | |
0.0066 | - |
Z-Glu(HMC)-Gly-OH | pH 8.0, 30°C | Cavia porcellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein glutamine + alkylamine | Cavia porcellus | - |
protein N5-alkylglutamine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | H0VXN6 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Cavia porcellus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | synthesis and kinetic characterisation of water-soluble fluorogenic acyl donors for transglutaminase 2, overview. Development of a fluorimetric TGase 2 activity assay with release of 7-hydroxycoumarin (HC) by TGase 2-catalysed hydrolysis or aminolysis of Z-Phe-gamma-Abu-HC resulting in a measureable increase in fluorescence. No activity with Z-Phe-gamma-Abu-7-amido-4-methylcoumarin and Z-Glu(AMC)-Gly-OH. Because the assay of TGase 2 requires the presence of an antioxidant to prevent oxidation of the active-site cysteine residue, both DTT and tris(2-carboxyethyl)phosphine (TCEP) are employed for investigations into the spontaneous reactivity of the compounds at a concentration of 0.5 mM. C-terminal extension with glycine results in kobs values that are increased by a factor of approximately four | Cavia porcellus | ? | - |
- |
|
peptide glutamine + aminoacetonitrile | - |
Cavia porcellus | ? | - |
? | |
peptide glutamine + N-(biotinyl)cadaverine | - |
Cavia porcellus | ? | - |
? | |
protein glutamine + alkylamine | - |
Cavia porcellus | protein N5-alkylglutamine + NH3 | - |
? | |
Z-Glu(HC)-Gly-OH + alkylamine | - |
Cavia porcellus | ? | - |
? | |
Z-Glu(HC)-Gly-OH + H2O | - |
Cavia porcellus | ? | - |
? | |
Z-Glu(HMC)-Gly-OH + alkylamine | best substrate | Cavia porcellus | ? | - |
? | |
Z-Glu(HMC)-Gly-OH + H2O | best substrate | Cavia porcellus | ? | - |
? | |
Z-Glu(HMC)-OH + alkylamine | - |
Cavia porcellus | ? | - |
? | |
Z-Glu(HMC)-OH + H2O | - |
Cavia porcellus | ? | - |
? | |
Z-Phe-gamma-Abu-7-hydroxy-4-methylcoumarin + alkylamine | - |
Cavia porcellus | Z-Phe-gamma-amino-N-alkylbutanamide + 7-hydroxy-4-methylcoumarin | - |
? | |
Z-Phe-gamma-Abu-7-hydroxy-4-methylcoumarin + H2O | - |
Cavia porcellus | Z-Phe-gamma-Abu-OH + 7-hydroxy-4-methylcoumarin | - |
? | |
Z-Phe-gamma-Abu-7-hydroxycoumarin + alkylamine | - |
Cavia porcellus | Z-Phe-gamma-amino-N-alkylbutanamide + 7-hydroxycoumarin | - |
? | |
Z-Phe-gamma-Abu-7-hydroxycoumarin + H2O | - |
Cavia porcellus | Z-Phe-gamma-Abu-OH + 7-hydroxycoumarin | - |
? | |
Z-Phe-Glu(HC)-Gly-OH + alkylamine | - |
Cavia porcellus | ? | - |
? | |
Z-Phe-Glu(HC)-Gly-OH + H2O | - |
Cavia porcellus | ? | - |
? | |
Z-Phe-Glu(HMC)-Gly-OH + alkylamine | - |
Cavia porcellus | ? | - |
? | |
Z-Phe-Glu(HMC)-Gly-OH + H2O | - |
Cavia porcellus | ? | - |
? | |
Z-Phe-Glu(HMC)-OH + alkylamine | - |
Cavia porcellus | ? | - |
? | |
Z-Phe-Glu(HMC)-OH + H2O | - |
Cavia porcellus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gpTGase 2 | - |
Cavia porcellus |
TGase 2 | - |
Cavia porcellus |
transglutaminase 2 | - |
Cavia porcellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cavia porcellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
Z-Phe-gamma-Abu-7-hydroxycoumarin | pH 8.0, 30°C | Cavia porcellus | |
0.32 | - |
Z-Glu(HMC)-Gly-OH | pH 8.0, 30°C | Cavia porcellus | |
0.33 | - |
Z-Glu(HC)-Gly-OH | pH 8.0, 30°C | Cavia porcellus | |
0.52 | - |
Z-Phe-gamma-Abu-7-hydroxy-4-methylcoumarin | pH 8.0, 30°C | Cavia porcellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
inhibition assay at | Cavia porcellus |
8 | - |
activity assay at | Cavia porcellus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics, irreversible enzyme inactivation usually results in steady-state rates that are equal to zero independent of inhibitor concentration | Cavia porcellus | |
0.00573 | - |
N-[(5S)-6-[4-(6-methylpyridin-2-yl)piperazin-1-yl]-6-oxo-5-(2-phenylacetamido)hexyl]prop-2-enamide | pH 6.5, 30°C, with/versus substrate Z-Glu(HMC)-Gly-OH | Cavia porcellus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
48.5 | - |
Z-Glu(HMC)-Gly-OH | pH 8.0, 30°C | Cavia porcellus | |
130.4 | - |
Z-Glu(HC)-Gly-OH | pH 8.0, 30°C | Cavia porcellus | |
204.1 | - |
Z-Phe-gamma-Abu-7-hydroxycoumarin | pH 8.0, 30°C | Cavia porcellus | |
456.1 | - |
Z-Phe-gamma-Abu-7-hydroxy-4-methylcoumarin | pH 8.0, 30°C | Cavia porcellus |