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Literature summary for 2.3.2.13 extracted from

  • Yang, M.T.; Chang, C.H.; Wang, J.M.; Wu, T.K.; Wang, Y.K.; Chang, C.Y.; Li, T.T.
    Crystal structure and inhibition studies of transglutaminase from Streptomyces mobaraense (2011), J. Biol. Chem., 286, 7301-7307.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces mobaraensis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 30% (w/v) PEG 8000, 50 mM NaCl, 1 mM EDTA, 1 mM 2-mercaptoethanol, 0.01% (w/v) NaN3, 100 mM cacodylic acid (pH 5.0), and 2% (v/v) glycerol Streptomyces mobaraensis

Protein Variants

Protein Variants Comment Organism
D301A the mutation drastically reduces the catalytic activity of the enzyme Streptomyces mobaraensis

Inhibitors

Inhibitors Comment Organism Structure
GLGQGETYR
-
 Streptomyces mobaraensis
SQAETQR
-
 Streptomyces mobaraensis
SQAETYR noncompetitive inhibition Streptomyces mobaraensis
SYAETYR noncompetitive inhibition Streptomyces mobaraensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
52.66
-
 Nalpha-benzyloxycarbonyl-L-glutaminylglycine in 250 mM Tris acetate buffer (pH 8.0), at 37°C Streptomyces mobaraensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Nalpha-benzyloxycarbonyl-L-glutaminylglycine + hydroxylamine Streptomyces mobaraensis
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces mobaraensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Streptomyces mobaraensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Nalpha-benzyloxycarbonyl-L-glutaminylglycine + hydroxylamine
-
 Streptomyces mobaraensis ?
-
 ?

Synonyms

Synonyms Comment Organism
MTGase
-
 Streptomyces mobaraensis
TGase
-
 Streptomyces mobaraensis
transglutaminase
-
 Streptomyces mobaraensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
35.48
-
 Nalpha-benzyloxycarbonyl-L-glutaminylglycine in 250 mM Tris acetate buffer (pH 8.0), at 37°C Streptomyces mobaraensis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.65
-
 in 250 mM Tris acetate buffer (pH 8.0), at 37°C Streptomyces mobaraensis SQAETYR
0.75
-
 in 250 mM Tris acetate buffer (pH 8.0), at 37°C Streptomyces mobaraensis SYAETYR

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.67
-
 Nalpha-benzyloxycarbonyl-L-glutaminylglycine in 250 mM Tris acetate buffer (pH 8.0), at 37°C Streptomyces mobaraensis