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Literature summary for 2.3.2.13 extracted from
- Purification and calcium dependence of transglutaminases from sheep hair follicles (1997), Biosci. Biotechnol. Biochem., 61, 1086-1090.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | 1 mM, 89% inhibition of DEAE-absorbed transglutaminase | Ovis aries |  |
| lysine | 1 mM, 43% inhibition of DEAE-unabsorbed transglutaminase, 90% inhibition of DEAE-absorbed transglutaminase | Ovis aries | |
| monoiodoacetate | 1 mM, 93% inhibition of DEAE-unabsorbed transglutaminase, 85% inhibition of DEAE-absorbed transglutaminase | Ovis aries | |
| N-ethylmaleimide | 1 mM, complete inhibition of DEAE-unabsorbed transglutaminase, 93% inhibition of DEAE-absorbed transglutaminase | Ovis aries | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | requirement | Ovis aries | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
2 * 40000, DEAE-absorbed transglutaminase, SDS-PAGE | Ovis aries |
| 77000 | - |
1 * 77000, DEAE-unabsorbed transglutaminase, SDS-PAGE | Ovis aries |
| 80000 | - |
DEAE-absorbed transglutaminase, gel filtration | Ovis aries |
| 82000 | - |
DEAE-unabsorbed transglutaminase, gel filtration | Ovis aries |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
Merino sheep | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-unabsorbed transglutaminase, CM-Sepharose, heparin-Sepharose, Superdex 200, DEAE-absorbed transglutaminase, DEAE-Sephacel, heparin-Sepharose, hydroxyapatite, Superdex 200 | Ovis aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hair follicle | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0052 | - |
DEAE-unabsorbed transglutaminase | Ovis aries |
| 0.092 | - |
DEAE-absorbed transglutaminase | Ovis aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein-bound gamma-glutamine + alkylamine | crosslinking of Hammersten casein, crosslinking between Ac-IB and Bz-Gly-Lys | Ovis aries | protein N5-alkylglutamine + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 40000, DEAE-absorbed transglutaminase, SDS-PAGE | Ovis aries |
| monomer | 1 * 77000, DEAE-unabsorbed transglutaminase, SDS-PAGE | Ovis aries |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Ovis aries |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Ovis aries |
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Release 2023.1 (January 2023)
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