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Literature summary for 2.3.2.13 extracted from

  • Tokunaga, F.; Yamada, M.; Miyata, T.; Ding, Y.L.; Hiranaga-Kawabata, M.; Muta, T.; Iwanaga, S.
    Limulus hemocyte transglutaminase. Its purification and characterization, and identification of the intracellular substrates (1993), J. Biol. Chem., 268, 252-261.
    View publication on PubMed
Search for more literature for 2.3.2.13

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol weak activation Tachypleus tridentatus

General Stability

General Stability Organism
loss of activity after freezing Tachypleus tridentatus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, complete inhibition Tachypleus tridentatus
EDTA 5 mM; 5 mM, complete inhibition Tachypleus tridentatus
hydroxylamine 100 mM, complete inhibition Tachypleus tridentatus
KCl above 500 mM Tachypleus tridentatus
monoiodoacetate 1 mM, 94% inhibition Tachypleus tridentatus
additional information not inhibited by diisopropylfluorophosphate Tachypleus tridentatus
N-ethylmaleimide 1 mM, 98% inhibition Tachypleus tridentatus
NaCl above 500 mM Tachypleus tridentatus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Tachypleus tridentatus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ requirement Tachypleus tridentatus
Ca2+ 8 mM, approx. 10fold activation Tachypleus tridentatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
 1 * 86000, SDS-PAGE Tachypleus tridentatus

Organism

Organism UniProt Comment Textmining
Tachypleus tridentatus
-
 japanese horseshoe crab
-

Purification (Commentary)

Purification (Comment) Organism
CM-Sepharose CL-6B, ammonium sulfate, DEAE-cellulose, Sephacryl S-300, DEAE-cosmogel, Zn-chelating Sepharose Tachypleus tridentatus

Source Tissue

Source Tissue Comment Organism Textmining
hemocyte
-
 Tachypleus tridentatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 938 amine incorporation units/min Tachypleus tridentatus

Storage Stability

Storage Stability Organism
4°C, at least 3 months Tachypleus tridentatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protein-bound gamma-glutamine + alkylamine identification of natural protein substrates Tachypleus tridentatus protein N5-alkylglutamine + NH3
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 86000, SDS-PAGE Tachypleus tridentatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Tachypleus tridentatus